Home    eJManager.com Add Your Journal   |    Follow on Twitter   |    Subscribe to List

Directory for Medical Articles
 

Open Access

-



Assessment of genetic diversity in Shorea robusta: an economically important tropical tree species

Giridara-Kumar Surabhi; Subhankar Mohanty; Rajesh Kumar Meher; Arup Kumar Mukherjee; Lakshmi Narayana R. Vemireddy.

Abstract
The present investigation reports an elucidation of genetic diversity among four-populations of most economically and ecologically important tree species, sal (Shorea robusta Gaertn.) for the first time in India, using ISSR markers. A total of one-hundred individual S. robusta trees were sampled from four different populations, considering twenty-five individuals from each population. In total, twenty-ISSR primers were screened with S. robusta DNA, and out of twenty, sixteen-primer produced reproducible amplicons. Sixteen selected ISSR markers were amplified a total of 118 alleles and the total number of amplicons for individual primers ranged from 5 to 12, with a mean of 7.37 alleles per primer, of which 74 were polymorphic with an average of 4.62 alleles per primer. The ISSR primer (GA)8YG yielded highest number of alleles (12) and primers (CA)8RG and (CT)8G yielded lowest number of alleles (5), with an average alleles size between 200-3500bp. The percentage of polymorphic alleles ranged from 40 [(CA)8RG] to 83.33 [(AC)8C]. A dendrogram based on UPGMA analysis grouped the four populations into two major clusters, having Keonjhar population into first cluster and rest three populations into second cluster. It was notable that the second major cluster was further divided into three-separate sub-clusters, representing population from each three locations. Analysis of molecular variance (AMOVA) revealed that the majority of genetic variation exists within populations, compared to the variation that exists among the populations. The present study is a clear-cut indication that inter- and intra-population genetic variation exists in S. robusta and ISSRs appears to be an efficient marker system in quantifying genetic variability in different populations of S. robusta.

Key words: AMOVA;genetic diversity;ISSR;molecular marker;polymorphism;Shorea robusta


Full text links

Share this Article




ScopeMed Home
Follow ScopeMed on Twitter
Article Tools
Job Opportunities/Service Offers
eJManager OJMS
eJPort Journal Hosting
About ScopeMed
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Service of eJManager LLC Publishing for Scientific Publications. Copyright © ScopeMedź Information Services.
Scopemed Buttons