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Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes

Tanya Prozorova; Alex Kamyshny; Vita Kamyshna.

Abstract. Using molecular genetic and immunofluorescence techniques we investigated the mRNA gene expression level of Aire, Deaf1, Foxp3, Ctla4 and Il10 in the offspring of rats with experimental gestational diabetes and in conditions of insulin oral tolerance formation. To determine the level of mRNA studied gene RT-PCR was performed in real time thermocycler CFX96 ™ Real-Time PCR Detection Systems («Bio-Rad Laboratories, Inc.», USA). We observed such violations of immunotolerance: AIRE gene repression, reduced mRNA levels of Deaf1 and the transcription factor Foxp3. This was accompanied by inhibition of gene expression suppressor cytokine Il-10 and negative costimulatory molecules Ctla4. Oral insulin during the first 2 weeks graded these changes, causing transcriptional activation of genes AIRE, Deaf1, Foxp3, Ctla4 and Il-10.

Key words: Experimental gestational diabetes;mesenteric lymph nodes;Aire;Deaf1;Foxp3

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