Home    eJManager.com Add Your Journal   |    Follow on Twitter   |    Subscribe to List

Directory for Medical Articles
 

Open Access

-



Detection of genotoxic effect of cadmium on tomato (Lycopersicon esculentum) using RAPD markers

Fatma A Asseri; Ahmed M Nada; Ashraf Haider.

Abstract
Tomato (Lycopersicon esculentum) seeds were germinated in Peteri dishes for 7-10 days. Seedlings were transferred to pots and grown in greenhouse for two weeks. Cadmium treatments were applied with water with concentrations 0.0, 10.0, 50.0, 70.0 and 100.0 µM. Genomic DNA from tomato leaves was used for RAPD-PCR analysis using four random primers (OPB05, OPB07, OPB08 and OPB09). RAPD profiles showed great variations in banding patterns, particularly in intensity, number, thickness and mobility of the generated PCR fragments. Appearance of new PCR products may be occurred because some oligonucleotide priming sites could become accessible to primers due to changes in DNA sequence. Disappearance of some DNA fragments might be due to structural rearrangement in DNA caused by different types of DNA damage after exposure to cadmium pollution which can change the number of binding sites of Taq polymerase. Structural rearrangement may also results in an increase in the intensity and thickness of some DNA fragments. The change in the mobility of some DNA fragments might be due to Single Strand Conformational Polymorphism (SSCP) that reveals differences in electrophoretic mobility between normal and mutant single strands of DNA. These results indicated that RAPD analysis could be used as a useful biomarker assay for detection of genotoxic effects of cadmium. Cadmium pollution can cause genotoxic effects and tomato plants can be used as a bioindicator for evaluation of environmental pollution by heavy metals.

Key words: genotoxicity;Cadmium;tomato;Lycopersicon esculentum;RAPD markers


Full text links

Share this Article




ScopeMed Home
Follow ScopeMed on Twitter
Article Tools
Job Opportunities/Service Offers
eJManager OJMS
eJPort Journal Hosting
About ScopeMed
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Service of eJManager LLC Publishing for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons