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IJCRR. 2017; 9(14): 01-02


Novel Application of Reversed Gel Electrophoresis: Reuse of DNA Molecular Weight Marker

Suhasa G.; Savithri Bhat.

Abstract
Gel electrophoresis is used in nucleic acid analysis and makes use of DNA/RNA molecular weight marker for size comparison. These size markers are used only once per electrophoresis and finally discarded along with the gel. In the present study, a reversed current is applied in agarose gel electrophoresis to reuse the DNA molecular weight marker, thus cutting down the cost involved in molecular experiments.

Key words: Agarose gel electrophoresis;DNA ladder;Nucleic acid


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