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Characterisation and Antibiotic Sensitivity of Staphylococcus aureus and Methicillin Resistant Staphylococcus aureus Isolated from Poultry Meat

Pankaj Patel; Pankaj Patel; Pankaj Patel; V. K.; B. Sunil; B. Sunil; B. Sunil; B. Sunil; Ebin Baby Mathews.

Abstract
The present study was undertaken to investigate the prevalence of Staphylococcus aureus and Methicillin resistant Staphylococcus aureus in poultry meat. A total of 58 poultry meat samples were collected from different retail markets of Kalpetta and Vythiri in Wayanad district, Kerala. Out of these 58 samples, 42(72.41%) were found positive for S. aureus by culture and these isolates were further confirmed by biochemical and molecular methods. The S. aureus isolates were further subjected to PCR to detect the prevalence of MRSA. None of the isolates were found to carry mecA gene. The obtained S. aureus isolates were subjected to antibiotic sensitivity test. The isolates were found highly sensitive to Chloramphenicol (93.10%), Cefuroxime (82.75%), Gentamicin (75.86%), Ciprofloxacin (75.86%), moderately sensitive to Erythromycin (48%) and found resistant to Methicillin, Vancomycin, Ampicillin, Amoxyclav, Co-trimoxazole and Tetracycline. This study confirmed the presence of S. aureus, especially antibiotic-resistant strains in poultry meat.

Key words: S. aureus;MRSA;Poultry Meat;Kerala


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