Spiders are one of the more diverse arthropod taxa, ranking seventh in global diversity, which makes them a fascinating group to study. The correct identification of many spider species is considered problematic due to complex variation in morphology. The Egyptian spider fauna are incompletely revised due to plenary studies on this group in Egypt from different localities. Samples of spiders were collected during 6 months (from 20/2/2016 till 2O/8/2016) from six different sites represented AL-Gharbia Province (30.881°N, 31.06°E) which is located in the north of the Arab Republic of Egypt about 90 km north of Cairo, and 120 km south east of Alexandria. Sampling of spiders using hand picking, sweep net and pitfall trap methods revealed total 157 specimens. A proposed key was designed to facilitate identification of the collected families, genera and species using their taxonomic characteristics of each. The present study results showed that, list of total 15 families, 19 genera and 15 species were recorded.