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Isolation and Identification of the Gut Microbiota of Healthy and Diarrhoeic Dogs in Andhra Pradesh, India

Noorbasha Mohammad Sharif; Bollini Sreedevi; Radha Krishna Chaitanya; Daggupati Sreenivasulu.

Abstract
Rectal swab samples from healthy (92) and diarrhoeic (44) dogs were analyzed in order to characterize canine gut microbiota. Isolation and identification of microbiota was carried out by conventional culture methods. Gut microbiota isolated include E. coli (65.2% incidence in healthy versus 75% in diarrhoeic dogs), Proteus (61.9% vs. 65.9%), Enterobacter (26% vs. 11.3%), Klebsiella (26% vs. 20.4%) and Pseudomonas (28.2% vs. 45.4%) species. E. coli isolates were further confirmed by PCR targeting E16S gene. Serological typing of PCR confirmed E. coli (93) revealed rough (19 isolates), O141 (5 isolates), O128 (2 isolates), O9 (2 isolates), O126 (2 isolates), O15, O20, O35, O49, O63, O85, O101, O116, O117, O118, O119 (1 isolate each) serotypes and 52 isolates were found to be ‘O’ group untypable (UT). The present study revealed the occurrence of Enteropathogenic (O49, O118, O119, O126, O128) and Enterotoxigenic (O15) strains of E. coli in dogs.

Key words: Dogs;Healthy and Diarrhoeic;Gut Microbiota



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