Home    eJManager.com Add Your Journal   |    Follow on Twitter   |    Subscribe to List

Directory for Medical Articles

Open Access


Isolation and Identification of the Gut Microbiota of Healthy and Diarrhoeic Dogs in Andhra Pradesh, India

Noorbasha Mohammad Sharif; Bollini Sreedevi; Radha Krishna Chaitanya; Daggupati Sreenivasulu.

Rectal swab samples from healthy (92) and diarrhoeic (44) dogs were analyzed in order to characterize canine gut microbiota. Isolation and identification of microbiota was carried out by conventional culture methods. Gut microbiota isolated include E. coli (65.2% incidence in healthy versus 75% in diarrhoeic dogs), Proteus (61.9% vs. 65.9%), Enterobacter (26% vs. 11.3%), Klebsiella (26% vs. 20.4%) and Pseudomonas (28.2% vs. 45.4%) species. E. coli isolates were further confirmed by PCR targeting E16S gene. Serological typing of PCR confirmed E. coli (93) revealed rough (19 isolates), O141 (5 isolates), O128 (2 isolates), O9 (2 isolates), O126 (2 isolates), O15, O20, O35, O49, O63, O85, O101, O116, O117, O118, O119 (1 isolate each) serotypes and 52 isolates were found to be ‘O’ group untypable (UT). The present study revealed the occurrence of Enteropathogenic (O49, O118, O119, O126, O128) and Enterotoxigenic (O15) strains of E. coli in dogs.

Key words: Dogs;Healthy and Diarrhoeic;Gut Microbiota

Full text links

Share this Article

ScopeMed Home
Follow ScopeMed on Twitter
Article Tools
Job Opportunities/Service Offers
eJManager OJMS
eJPort Journal Hosting
About ScopeMed
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Service of eJManager LLC Publishing for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons