Home    eJManager.com Add Your Journal   |    Follow on Twitter   |    Subscribe to List

Directory for Medical Articles

Open Access


Duplex PCR for Direct Detection of Mycoplasma gallisepticum and Mycoplasma synoviae from Tissues of Poultry Affected with Respiratory Infections

Piyush Tomar; Y. Singh; N.K. Mahajan; Naresh Jindal; Mahavir Singh.

In the present study, 92 pooled tissue samples including trachea, lungs and air sacs were collected from 92 different poultry flocks affected with respiratory infections and were subjected to mycoplasmological examination by duplex PCR. Result of duplex PCR revealed, 25/92 (27%) MG, 2/92 (2.1%) MS and 1 (1.08%) sample was positive for both MG and MS. In conclusion, duplex PCR was found to be a better assay to detect two most pathogenic avian mycoplasmas (M. gallisepticum and M. synoviae) simultaneously directly from poultry tissue sample and also could serve as a sensitive, specific and rapid tool in monitoring and surveillance programmes on poultry farms in Haryana. It seems to be the first study of this type in India.

Key words: Mycoplasma gallisepticum;Mycoplasma synoviae;Molecular diagnostic;Duplex PCR;16S rRNA;Intergenic Spacer Region

Full text links

Share this Article

ScopeMed Home
Follow ScopeMed on Twitter
Article Tools
Job Opportunities/Service Offers
eJManager OJMS
eJPort Journal Hosting
About ScopeMed
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Service of eJManager LLC Publishing for Scientific Publications. Copyright © ScopeMedź Information Services.
Scopemed Buttons