Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

-



Alizarin Red-S Skeletal Preparation of Adult Red-Vented Bulbul Bird (Pycnonotus cafer)

Raja Raviteja Ersavadla; Rajendranath Nalla.

Abstract
Alizarin Red-S preparation was done for preparing museum specimens of one dead Red-vented Bulbul Passerine bird (Pycnonotus cafer) retrieved from college premises. For clear visibility of the skeleton, specimen of Bulbul bird was de-feathered, de-skinned and eviscerated followed by fixing in 10 per cent Neutral buffered formalin for a week. Post fixation, the specimen was stained in Alizarin Red-S until bone stains red and excess stain was removed by fresh potassium hydroxide solution. Later, specimen was cleared in solutions containing glycerin, 2 per cent potassium hydroxide and distilled water for a month. Results showed a clear red stained skeleton due to affinity of Alizarin Red-S stain to calcium salts. Stained specimen was cleaned neatly and mounted into museum jar containing pure glycerol and few thymol crystals.

Key words: Alizarin red-s;Potassium hydroxide;Red-vented Bulbul



Share this Article


Advertisement
American Journal of Physiology, Biochemistry and Pharmacology

SUBMIT YOUR ARTICLE NOW




ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Article Tools
eJPort Journal Hosting
About ScopeMed
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons