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Effect of Oxidative Stress on Glutathione Reductase Activity of Escherichia coli Clinical Isolates from Patients with Urinary Tract Infection

Fariha Akhter Chowdhury; Sabrina Mahboob; Anamika Saha; Afrin Jahan; Mohammad Nurul Islam.

Abstract
Urinary tract infection (UTI) is frequently encountered by female population where the episode of occurrence increases with advancing age. Escherichia coli, a common UTI causing organism, retains glutathione defense mechanisms that may allow the organism to withstand host oxidative immune response as well as the harsh physiological environment of urinary tract. The aim of this study was to analysis glutathione reductase (GR) activity of UTI causing E. coli under stressful condition. E. coli isolates from urine samples of UTI female patients of different ages were sampled. Samples of isolated E. coli were grown in conditions with and without oxidative stress where the stressful condition was induced by hydrogen peroxide (H2O2). In both conditions measurement of reduced glutathione level of the isolates was performed calorimetrically using microplate reader assay. Samples from elderly patients showed that GSH level significantly altered, the value of GSH (mM/mg) are 5.01±0.48 and 5.20±0.64 in normal and under stressful condition respectively, which showed increase of GSH level by 3.83% (p = 0.024) under oxidative stress condition. On the other hand, in case of adult patients, GSH (mM/mg) level found to be decreased by 5.11% (p = 0.011) with the values of 5.08±0.1 and 4.82±0.18 respectively for normal and stressed condition. Our data suggesting an association of GR activity with patient’s age, may signify that E. coli isolates respond differently to oxidative stressful environme

Key words: Escherichia coli;glutathione reductase;oxidative stress;reduced glutathione (GSH);Urinary tract infections (UTI)



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