Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Research



Detection of Vibrio parahaemolyticus From Marine Crabs in and Around Puducherry and Its Public Health Significances

Vijayan Chakarapani; Ajaykumar Vattiringal Jayan; Bhattacharya Anjan; Bhanurekha Vivekanandan.

Abstract
ABSTRACT
Food borne illnesses have major social and economic impacts. The WHO estimates that worldwide food borne and waterborne diarrheal diseases together kill about 2.2 million people annually and in India around 6 per cent of the population. Vibrio parahaemolyticus has recently been recognized as one of the most important food borne pathogens as the leading causal agent of human acute gastro enteritis. This study was conducted to detect the presence of V. parahaemolyuticus from marine crabs sold in puducherry and human stool samples and was isolated as per the standard procedure. A total of 35 marine crab samples and 50 human stool samples were screened for presence of V. parahaemolyticus. Out of 35 samples, 14 samples (40 per cent) and out of 50 stool specimens, only 2 (4 per cent) were positive for V. parahaemolyticus by conventional methods. This isolates were further subjected to PCR. Out of 18 isolates, 14 isolates (87.5 per cent) from crabs and 100 per cent from stool specimens of V. parahaemolyticus were confirmed and subjected to biofilm production assay by CRA plate method. In that 14 isolates, 9 were positive for biofilm production and none of the isolates from human stool samples were positive for biofilm production. The antibiotic resistance profiling of the V. parahaemolyticus isolates does not show high resistance. This can be a major concern as microorganisms can be associated with chronic and recurrent human infections. This study clearly indicates the need for proper handling and processing of seafood especially in marine crabs. It is also important that in kitchen when ever seafood is prepare, care shall be taken to avoid cross contamination.

Key words: Vibrio parahaemolyticus;Marine Crabs;Biofilm;Puducherry;Food Borne



Share this Article


Advertisement
American Journal of Diagnostic Imaging

SUBMIT YOUR ARTICLE NOW




ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Article Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons