A total of thirty two E.coli isolates obtained from poultry intestines were subjected to phenotypic screening of Extended Spectrum Betalactamase (ESBL) and AmpC Betalactamase resistance by novel disc placement method involving both inducer and indicator discs. Among them 14 isolates were indicative of ESBL producers and two were identified as AmpC derepressed mutants. They were also subjected for slime production by Modified Congo Red Agar (MRCA) method and found all were positive by producing dry crystalline black coloured colonies. The simplex PCR was standardized for three genes (TEM, SHV, CTX-M) to run at a same annealing temperature 60°C for 60 sec. The genotypic screening revealed none of the 32 isolates were positive for the above genes. Identification of antibiotic resistance and presence of biofilm producing ESBL E.coli in faeces of poultry origin indicates zoonotic and public health significance which emphasizes routine screening of poultry meat thereby appropriate strategies can be adopted to combat this resistance.