Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Research

Detection and Quantification of Bluetongue Virus Serotype 23 by Evagreen Based Real Time PCR

Shabir Ahmad Bhat; Jaynudin Khorajiya; Bilal Ahmad Malla; Pervaiz Ahmad Dar; Karam Pal Singh; Sukdeb Nandi; Mudasir Ahmad.

Bluetongue, an economically important arthropod-borne viral disease of livestock especially sheep. Presently, there are 27 serotypes of bluetongue virus prevalent worldwide. Traditional bluetongue virus typing methods such as virus isolation and serum neutralization tests (SNT) are cumbersome, time-consuming, and many times inconclusive. The other diagnostic methods such as antigen-capture enzyme-linked immunosorbent assay (ELISA), dot immunobinding assay, and immuno-electron microscopy, have been developed. All these techniques have limited sensitivities and thus are unable to detect the virus at a sub-clinical level. Serotype specific RT-PCR and qRT-PCR based amplification of BTV Seg-2 are most reliable, less time consuming and high specificity for serotype typing. In the present study, standardization of EVA-green based qRT-PCR was done for amplification and quantification of BTV-23 serotype from infected BHK-21 cell culture; the results showed high sensitivity as well specificity of realtime PCR for diagnosis of BTV-23.

Key words: Bluetongue virus;Serotype;EVA-green;Cell culture

Share this Article

American Journal of Preventive Medicine and Public Health


ScopeMed Home
Follow ScopeMed on Twitter
Article Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons