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In vitro evaluation of antibacterial activity of total protein-assisted extractions of some invertebrates against human pathogenic bacteria

Mohamed Hassan Mona; El Sayed Taha Rizk; Wesam Mohamed Salama; Mohamed Nassef; Mai Lotfy Younis.

Abstract
The present study aims to evaluate the In vitro antibacterial activity of invertebrate total protein-assisted extractions. To achieve this goal, tissues samples collected from molluscans (Lithophaga lithophaga and Pinctada radiate) and crustaceans (Portunus pelagica) were homogenized and total proteins were extracted by Tris Buffer method. In vitro antibacterial activity of extracted total proteins against two gram-negative bacterial strains (Escherichia coli and Pseudomonas aeruginosa) and two gram-positive bacterial strains (Staphylococcus aureus and Streptomyces sp.) was measured using disc diffusion assays. As a result, comparing to their effects in the control, the total protein extracts, at their low and high doses, had a significant effects (P ≤ 0.05) on the four bacterial strains with the observation that in all protein extracts, the high dose was higher in the antibacterial effect (mean= 1.33) than the low dose extracts (mean= 1.14). Additionally, the highest antibacterial activity was recorded in P. radiata protein extracts (mean=1.49), followed by P. pelagicus protein extracts (mean= 1.44) and finally, the L. lithophaga total protein extracts. To conclude, total protein-assisted extractions from marine invertebrates: L. lithophaga, P. radiata and P. pelagica could be used as an effective antibacterial material and further studies are needed to evaluate their biological and pharmaceutical mechanistic approaches.

Key words: Marine invertebrates;Protein extraction;In vitro;Antibacterial effects



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