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Identification of a Common Protein Moiety of Aeromonas Strains Using Western Blot Technique

N. Sachan, R. K. Agarwal, V. P. Singh.

Abstract
Aeromonads are common contaminants in foods such as fish and other sea foods, raw and cooked meat, poultry, vegetables, milk and milk products. These organisms are responsible for several extraintestinal human infections such as meningitis, endocarditis, peritonitis, osteomyelitis, septic arthritis, septicaemia, eye and urinary tract infections. Keeping the importance of these organisms in mind present study was envisaged to develop immunoassays for efficient and rapid detection of Aeromonas spp. vis-a-vis identification of a common polypeptide moiety in their outer membrane proteins (OMP).Western blot study employing anti-OMP serum indicated polypeptides of 25 and 35 kDa to be immunogenic in most of the Aeromonas strains. The findings of this study will be of immense value in surveillance and monitoring of Aeromonas spp. in foods.

Key words: Aeromonas, OMP, SDS-PAGE, western blot, foods.


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