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Spatula DD
Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery
Periodical of DD
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ISSN: 1309-9914 (Print)
Language: [ Turkish ]   [ English ]  
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  » Volume 4, Issue 2
      pp. 55-119
  » Volume 4, Issue 1
      pp. 1-53

 

Open Access

Original Research



Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen

Ahmed Mohamed Ahmed Abd El-Mawla, Husam Eldien Husien Osman.

Abstract
ABSTRACT
AIM: Breast cancer remains the most common malignancy in women world wide. Estrogen levels appear to be associated with an increased risk for the development of breast cancer. Tamoxifen (TAM), a non steroidal antiestrogen, is used as a chemotherapeutic and chemopreventive agent for breast cancer. Propolis has been reported to be important antioxidant. This study aims to elucidate the protective effects of propolis on rat liver after treatment with TAM.
METHODS: Three groups of adult female albino rats were used; each containing 10 rats. Group 1 (control): rats were administrated with 0.9% NaCl. Group 2 (experimental): rats were received a diet with 20 mg/kg bw TAM. Group 3 (experimental): rats received a diet containing propolis (50 mg/kg bw) prior to daily TAM 20 mg/kg bw. Paraffin sections were used for histopathological studies.
RESULTS: Histopathological degenerative effects in the form of vacuolar degeneration, fatty changes and hydropic degeneration were noticed in TAM treated rat liver. Karyolysis and karyorrhexis were also seen. Dysplasia and chromatin clumping were observed in scattered hepatocytes. Histopathological and ultrastructural changes were diminished in rats treated with propolis prior to TAM.
CONCLUSION: The present study noticed the protective effect of propolis in improving the histological and ultrastructural changes of liver after treatment with TAM.

Key words: Keywords: Propolis, tamoxifen, antiestrogen, breast cancer, antioxidant, liver.



Tamoksifen tedavisi sonrası karaciğerdeki histolojik ve ultrastrüktürel değişikliklerin geliştirilmesinde propolisin rolü

Özet
AMAÇ: Meme kanseri dünya çapında kadınlarda en sık görülen malignite olarak kalmıştır. Östrojen seviyeleri, meme kanseri gelişimi için artmış bir riskle ilişkili olduğu görülmektedir. Nonsteroidal bri antiöstrojen olan tamoksifen (TAM), meme kanseri için bir kemoterapötik ve kemopreventif olarak kullanılmaktadır. Propolisin önemli bir antioksidan olduğu rapor edilmiştir. Bu çalışma, TAM ile tedavi sonrasında sıçan karaciğerleri üzerindeki propolisin koruyucu etkilerini değerlendirmektir.
YÖNTEM: Herbirinde 10 sıçan bulunan 3 grup erişkin dişi sıçan kullanıldı. Grup 1 (kontrol): sıçanlara % 0,9 NaCl uygulandı. Grup 2 (deneysel): sıçanlara 20 mg/kg dozunda TAM verildi. Grup 3 (deneysel): Sıçanlara 20 mg/kg TAM öncesinde propolis (50 mg/kg vücut ağırlığına) içeren bir diyet verildi. Histopatolojik çalışmalar için parafin kesitler kullanıldı.
BULGULAR: Tamoksifen uygulanmış ratların karaciğerlerinde vakuolar dejenerasyon, yağlı değişiklikler ve hidropik dejenerasyon şeklinde histopatolojik dejeneratif etkiler fark edildi. Ayrıca karyoliz ve karyoreksis gözlendi. Dağınık hepatositlerde displazi ve kromatin topaklanması gözlendi. TAM öncesinde propolis verilen sıçanlarda histopatolojik ve ultrastrüktürel değişiklikler azalmıştı.
SONUÇ: Çalışmamız, TAM uygulaması sonrasında karaciğerdeki histolojik ve ultrasrüktürel değişikliklerin düzeltilmesinde propolisin koruyucu etkisini gösterdi.

Anahtar Kelimeler: Propolis, tamoksifen, antiöstrojen, meme kanseri, antioksidan, karaciğer.



REFERENCES
1. Hay Goss PE, Stresses-Weipple K. Aromatase inhibitors for chemoprevention. Best Pract. Res. Clin. Endocrinol. Metab. 2004; 18 (1): 113-130.
[DOI via Crossref]   

2. Dray X, Tainturier MH, De La Lande P, Marty O, Mallet L. Cirrhosis with non alcoholic steatohepatitis: role of tamoxifen. Gastroenterol. Clin. Biol. 2000; 24(11): 1122-1123.
[
Pubmed]   

3. Kim SY, Suzuki N, Laxmi YR, Umemoto A, Matsuda T, Shibutani S. Antiestrogens and the formation of DNA damage in rats: A comparison. Chem. Res. Toxicol. 2006; 19(6): 852-858.
[
DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   

4. Hamada N, Ogawa Y, Saibara T, Murata Y, Kariya S, Nishioka A, et al. Toremifene-induced fatty liver and NASH in breast cancer patients with breast-conservation treatment. Int. J. Oncol. 2000; 17(6):1119-1123.
[
Pubmed]   

5. Curtis RF, Freedman DM, Sherman MF, Fraumeni JF. Risk of maliganant mixed mullerian tumors after tamoxifen therapy for breast cancer. J. Nat. Cancer Inst. 2004; 7, 96 (1): 70-74.


6. Decensi A, Bonanni B, Guerrieri-Gonzaga A, Gandini S, Robertson C, Johansson H, et al. Biologic activity of tamoxifen at low doses in healthy women. J. Natl. Cancer. Inst. 1998; 90: 1461-1467.
[
DOI via Crossref]   

7. Srinivas GAnnab LA, Gopinath G, Banerji A, Srinivas P. Antisense blocking of BRCAI enhances sensitivity to plumbagin but not tamoxifen in BG-1 ovarian cancer cells. Mol. Carcinogen. 2004; 39 (1): 15-25.
[
DOI via Crossref]    [Pubmed]   

8. Newboid RR, Jefferson WN, Padilla Burgos E. Neonatally treated with tamoxifen. Carcinogenesis (Lond), 1997; 18: 2293-2298.
[
DOI via Crossref]    [Pubmed]   

9. Divi RL, Dragam YP, Pitot HC, Poirler MC. Immunohistochemical localization and semiquantitation of hepatic tamoxifen DNA adducts in rats exposed orally by tamoxifen. Carcinogenesis, 2001; 22 (10): 1693-1699.
[
DOI via Crossref]    [Pubmed]   

10. Hirsimaki P, Hirsimaki Y, Neiminen L and Joe-Payne B. Tamoxifen induced hepatocellular carcinoma in rat liver: A-1 year study with antiestrogen. Arch. Toxicol. 1993; 67: 49-54.
[
DOI via Crossref]   

11. Kasahara T, Hashiba M, Harada T, Degawa M. Change in gene expression of hepatic tamoxifen metabolizing enzymes during the process of tamoxifen induced hepatocarcinogenesis in female rats. Carcinogenesis, 2002; 23 (3): 491-498.
[
DOI via Crossref]    [Pubmed]   

12. Eisner A, Austin DF and Samples JR. Short wave length automated perimetry and tamoxifen uses. Br. J. Ophtalmol. 2004; 88 (1): 125-130.
[
DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   

13. Epstein MD, Samuel S, David S. The Breast Cancer Prevention program. Macmillan, New York. 1997; 145.


14. Dsouza UJ. Tamoxifen induces multinucleated cells (symplasts) and distortion of seminiferous tubules in rats' testes. Asian J. Androl. 2003; 5 (3): 217-220.
[
Pubmed]   

15. Sforcin JM, Fernandes A, Lopes CAM, Bankova V, Funari SRC. Seasonal effect on Brazilian propolis antibacterial activity. J. Ethnopharm. 2000; 73: 243-249.
[
DOI via Crossref]   

16. Khayyal MT, El-Ghazaly MA, El-Khatib AS. Mechanisms involved in the anti-inflammatory effect of propolis extract. Drugs Exp. Clin. Res. 1993; 19: 197-203.
[
Pubmed]   

17. Bazo AP, Rodrigues MAM, Sforcin JM, Camargo JLV, Ribeiro LR, Salvadori DMF. Protective action of propolis on the rat colon carcinogenesis. Teratog. Carcinog. Mutag. 2002; 22: 183-194.
[
DOI via Crossref]    [Pubmed]   

18. Jasprica D, Mornar A, Debeljak Z, Smolcic-Bubalo A, Medic-Saric M, Mayer L, et al. In vivo study of propolis supplementation effects on antioxidative status and red blood cells. J. Ethnopharmacol. 2007; 110: 548-554.
[
DOI via Crossref]    [Pubmed]   

19. Kanbura M., Eraslan G., Silici S., (2009): Antioxidant effect of propolis against exposure to propetamphos in rats. Ecotoxicol. Environ. Safety 72, 900-915.


20. Gonzalez R, Rernirez D, Rodriguez S. Hepatoprotective effects of propolis extract on paracetarnol induced liver damage in mice. Phytother. Res. 1994; 8: 229-232.
[
DOI via Crossref]   

21. Sforcin JM, Kaneno R, Funari SRC. Absence of seasonal effect on the immunomodulatory action of Brazilian propolis on natural killer activity. J. Venom. Animals Toxins, 2002; 8: 19-29.
[
DOI via Crossref]   

22. Khalil ML. Biological activity of bee propolis in health and disease. Asian Pac. J. Cancer Prev. 2006; 7: 22-31.
[
Pubmed]   

23. Yousef MI, Kamel IK, Esmail AM, Baghdadi HH. Antioxidant activities and lipid lowering effects of isoflavone in male rabbits. Food Chem. Toxicol. 2004; 42: 1497-1503.
[
DOI via Crossref]    [Pubmed]   

24. Mani F, Damasceno HC, Novelli EL, Martins EA, Sforcin JM. Propolis: effect of different concentrations, extracts and intake period on seric biochemical variables. J. Ethnopharmacol. 2006; 105: 95-98.
[
DOI via Crossref]    [Pubmed]   

25. Abd El-Mawla AMA, Osman HEH. HPLC analysis and role of the Saudi Arabian propolis in improving the pathological changes of kidney treated with monosodium glutamate. Spatula DD, 2011; 1(3): 119-127.
[
DOI via Crossref]   

26. Castaldo S, Capasso F. Propolis, an old remedy used in modern medicine. Fitoterapia, 2002; 73: 1-6.
[
DOI via Crossref]   

27. Drury RAB, Wallington FA. Corleton’s Histological Technique 4th Ed. Oxford, New York, Toronto, Oxford university press. 1980.


28. Bancroft JD, Gamble M. Theory and Practice Histological Techniques, 5th ed., Churchill Livingstone. New York, Edinburgh and London, 2002; 126: 173-175.


29. Maronpot RR, Fox T, Malarkey DE, Goldsworthy TL. Mutations in rats Photo-oncogene: clues to etiology and molecular pathogenesis of mouse liver tumors. Toxicology, 1995; 101: 125-156.
[
DOI via Crossref]   

30. Sismondi P, Biglia N, Volpi E, Giai M, Grandis T. Tamoxifen and the endometrium. The human endometrium. (Eds. Bulletti C, Gurpide E, Flamigni C) The New York Academy of Sciences New York, 1994; 10-321.


31. Bush TL, Helzlsouer K. Tamoxifen for the primary prevention of breast cancer: A review and critique of the concept and trial. Epidemiologic Reviews, 1993; 15 (1): 233-43.
[
Pubmed]   

32. Wolf DM, Jordan VC. Gynecologic complications associated with longterm adjuvant tamoxifen therapy for breast cancer. Gynecol Oncol. 1992; 45: 118-128, 1992.
[
DOI via Crossref]   

33. Lahli L, Blanco G, Kauppila A. Endometrial changes in postmenopausal breats cancer patients receiving TAM. Obstet & Gynecol. 1992; 79: 111-1116.
[
Pubmed]   

34. Wullaert A, Wielockx B, vanHuffel S, Bogaert V, Degest V, Papeleu P, et al. Adenoviral gene transfer of ABIN-1 protects mice from TNF galactosamine-induced acute liver failure and lethality, Hepatology, 2005; 42 (2): 381.
[
DOI via Crossref]    [Pubmed]   

35. El-Beshbishy HA. Lipoic acid attenuates DNA fragmentation, oxidative stress and liver injury induced by tamoxifen in rats, Asian J Trad Med. 2007; 2 (5): 175.


36. El-Beshbishy HA, Mohamadin AM, Nagy AA, Abdel-Naim AB. Amelioration of tamoxifen-induced liver injury in rats by grape seed extract, black seed extract and curcumin. Indian Journal of Experimental Biology, 2010; 48: 280-288.
[
Pubmed]   

37. Hirsimaki P, Hirsimaki Y, Neiminen L, Joe-Payne B. Tamoxifen induced hepatocellular carcinoma in rat liver: A-1 year study with antiestrogen. Arch. Toxicol. 1993; 67: 49-54.
[
DOI via Crossref]   

38. Kasahara T, Hashiba M, Harada T and Degawa M. Change in gene expression of hepatic tamoxifen metabolizing enzymes during the process of tamxifen induced hepatocarcinogenesis in female rats. Carcinogenesis, 2002; 23 (3): 491-498.
[
DOI via Crossref]    [Pubmed]   

39. Badawy SA, El-Far FI and Amer HA. Testicular and post testicular role of estrogen in adult male rabbit. Egypt. J. Basic and Appl. Physiol. 2002; 1(2): 269-280.


40. Morsy FA, Amina Gamal el Din, Nermeen M. Shaffie, Manal A, Badawi. Histopathologic study of Tamoxifen Induced Liver Damage in Rats and Vitamins Ameliorative Effect. Nature and science, 2010; 8 (5): 1-15.


41. Higgins CM, Jung C, Ding H, Xu Z. Mutant Cu, Zn supreoxide dismutase that causes motonuron degeneration is present in mitochondria in the CNC. J. Neuro. Sci. 2003; 22: 215.


42. Jaarsma D, Rognoni F, Van Dugn W. Cu Zn superoxide dismutase (SODI) accumulates in vacuolated mitochondria in transgenic mice expression amyotrophic lateral sclerosis linked SODI mutations. Acta. Neuropathol. 2001; 102: 293-305.
[
Pubmed]   

43. Wiedemann FR, Manfredi G, Mawrin C. Mitochondrial DNA and respiratory chain function in spinal cords of ALS patients. J.Neurochem. 2002; 80: 616-625.
[
DOI via Crossref]    [Pubmed]   

44. Bruno S, Maisonneuve P, Castellana P, Rotmensz N, Rossi S, Maggioni M, et al. Incidence and risk factors for non-alcoholic steatohepatitis: prospective study of 5408 women enrolled in Italian tamoxifen chemoprevention trial. Br. Med. J. 2005; 330: 932.
[
DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   

45. Liu CL, Huang JK, Cheng SP, Chang YC, Lee JJ, Liu TP. Fatty liver and transaminase changes with adjuvant tamoxifen therapy. Anticancer Drugs, 2006; 17(6):709-713.
[
DOI via Crossref]    [Pubmed]   

46. Larosche I, Lettéron P, Fromenty B, Vadrot N, Abbey-Toby A, Feldmann G, et al. Tamoxifen Inhibits Topoisomerases, Depletes Mitochondrial DNA, and Triggers Steatosis in Mouse Liver. Journal of Pharmacology and Experimental Therapeutics (JPET), 2007; 321: 526-535.
[
DOI via Crossref]    [Pubmed]   

47. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J.Food Drug Analaysis, 2002; 10: 178-182.


48. Gómez-Caravaca AM, Góomez-Romero M, Arráez- Román D, Segura-Carretero A, Fernández-Gutiérrez A. Advances in the analysis of phenolic compounds in products derived from bees. J. Pharm. Biomed. Anal. 2006; 41: 1220-1234.
[
DOI via Crossref]    [Pubmed]   

49. Mokhtar IY, Afrah FS. Propolis protection from reproductive toxicity caused by aluminium chloride in male rats. Food and Chemical Toxicology, 2009; 47(6): 1168-1175.
[
DOI via Crossref]    [Pubmed]   

50. El-Masry Thanaa A, Ashraf ME, Nagla AE. Possible protective effect of propolis against lead-induced neurotoxicity in animal model. Journal of Evolutionary Biology Research, 2011; 3 (1): 4-11.


51. Hazel ER, Helen MG. The effect of the flavonoids, quercetin, myricetin and epicatechin on the growth and enzyme activities of MCF7 human breast cancer cells Chemico-Biological Interactions, 1998; 116 (3): 213-228.
[
DOI via Crossref]   

52. Radhakrishnan P, Palaniyandi S, Hanapal S. Therapeutic effect of propolis and paclitaxel on hepatic phase I and II enzymes and marker enzymes in dimethylbenz (a) anthracene-induced breast cancer in female rats. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2006; 143 (3): 349-354.
[
DOI via Crossref]   

How to Cite this Article
Pubmed Style

El-Mawla AMAA, Osman HEH. Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen. Spatula DD. 2012; 2(1): 35-42. doi:10.5455/spatula.20120322073620



Web Style

El-Mawla AMAA, Osman HEH. Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen. www.scopemed.org/?mno=16891 [Access: November 23, 2014]. doi:10.5455/spatula.20120322073620



AMA (American Medical Association) Style

El-Mawla AMAA, Osman HEH. Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen. Spatula DD. 2012; 2(1): 35-42. doi:10.5455/spatula.20120322073620



Vancouver/ICMJE Style

El-Mawla AMAA, Osman HEH. Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen. Spatula DD. (2012), [cited November 23, 2014]; 2(1): 35-42. doi:10.5455/spatula.20120322073620



Harvard Style

El-Mawla, A. M. A. A. & Osman, H. E. H. (2012) Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen. Spatula DD, 2 (1), 35-42. doi:10.5455/spatula.20120322073620



Turabian Style

El-Mawla, Ahmed Mohamed Ahmed Abd, and Husam Eldien Husien Osman. 2012. Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen. Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery, 2 (1), 35-42. doi:10.5455/spatula.20120322073620



Chicago Style

El-Mawla, Ahmed Mohamed Ahmed Abd, and Husam Eldien Husien Osman. "Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen." Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery 2 (2012), 35-42. doi:10.5455/spatula.20120322073620



MLA (The Modern Language Association) Style

El-Mawla, Ahmed Mohamed Ahmed Abd, and Husam Eldien Husien Osman. "Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen." Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery 2.1 (2012), 35-42. Print. doi:10.5455/spatula.20120322073620



APA (American Psychological Association) Style

El-Mawla, A. M. A. A. & Osman, H. E. H. (2012) Role of propolis in improving the histological and ultrastructural changes of liver after treatment with tamoxifen. Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery, 2 (1), 35-42. doi:10.5455/spatula.20120322073620



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