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Vet World. 2010; 3(8): 364-366

Assessment of Escherichia coli isolates for In vitro biofilm production

A.I. Dadawala, Chauhan H.C., Chandel, B.S., Ranaware, P., Patel Sandip S, Khushboo Singh, Rathod P.H., Shah N.M. and Kher, H.N..

A total of 14 Escherichia coli isolates were assessed for their ability to produce biofilm in-vitro by
slime production on Congo red agar medium (CRA) and microtitre plate assay. Out of 14 isolates
tested, 12 were slime producing on CRA as indicated by black colonies. The isolates of E.coli varied
in their ability to produce biofilm on the surface of microtitre plate ranging from 0.101 to 0.543 ODm.
Out of 14 isolates tested, 10 were positive for biofilm production employing criterion of blank
corrected ODs9s > 0.1. Two of slime negative isolated were also negative for biofilm production
where as the two slime positive isolates were found to be negative for biofilm production.

Key words: Biofilm, E.coli, Slime production, Microtitre plate assay, In vitro.

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