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Vet World. 2010; 3(8): 375-377


Isolation, identification and molecular characterization of IBV variant from out break of visceral gout in commercial broilers

Amit Gaba, Hanish Dave, J.K.Pal and K.S.Prajapati.

Cited by (1)

Abstract
Kidney tissue samples of field outbreak of visceral gout in commercial broilers from Anand District
of Gujarat, India were processed for virus isolation, identification and molecular characterization.
Isolates produced typical embryo lesions of curling and stunting suggestive of infectious bronchitis
after three passages. RT-PCR and sequence analysis was carried out from infected allantoic fluid.
The nucleotide sequence was found to have 99% nucleotide sequence identity with avian infectious
bronchitis virus strain 4/91(pathogenic). Phylogenetic analysis using Clustal W program, the virus
was found to group with 793/B and 4/91 strain of viruses.

Key words: Molecular, Virus, Commercial Broiler, Visceral Gout, Outbreak, Mortality



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