This study was conducted in order to find out the effect of air space on the top of glass vial in which semen is
stored, on the motility of spermatozoa. 45 samples collected from two bucks over a span of 6 months were used
for experiment. Goat milk extender was the diluent used. Two ml each of diluted semen after noting their initial
motility was stored in 2 ml and 5 ml vials. Samples were stored at 5°C and motility of spermatozoa noted at 24
and 48 hours. Semen without air space was found to preserve the motility better than semen with air space on
24 and 48 hours of incubation. This could be better attributed to reactive oxygen species production by the
spermatozoa, but further investigation is needed in this aspect to confirm it.
Sperm Motility, Oxidative Stress, Buck Semen, Reactive Oxygen Species