Home    eJManager.com Add Your Journal   |    Follow on Twitter   |    Subscribe to List

Directory for Medical Articles
 

Open Access

Original Research

Vet World. 2010; 3(9): 414-416


Isolation, identification and molecular characterization of Inclusion body hepatitis virus

Amit Gaba, Hitesh Parmar, J.K.Pal and K.S.Prajapati*1.

Abstract
Inclusion body hepatitis caused by avian adenovirus with highly pathogenic strain lead to heavy mortality and
unusual lesion like hydropericardium in commercial broilers. Samples were collected from an outbreak in
12000 broiler flock from Andhra Pradesh with sudden high mortality initiated from 32 days of age. Liver was
enlarged and showed petechae. Hydropericardium was extensive. 10% liver suspension inoculated to SPF
chicks produced mortality with hydropericardium and showed presence of intranuclear inclusions in the
hepatocytes. Viral DNA extracted from infected liver processed for PCR produced an amplicon of 890 bp.
Nucleotide sequence analysis using blast programme of NCBI showed 99% identity with fowl adenovirus.

Key words: Inclusion body, Hepatitis, Avian Adenovirus, Broiler, Vaccine, Molecular Characterization.



Share this Article




ScopeMed Home
Follow ScopeMed on Twitter
Article Tools
Job Opportunities/Service Offers
eJManager OJMS
eJPort Journal Hosting
About ScopeMed
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Service of eJManager LLC Publishing for Scientific Publications. Copyright © ScopeMedź Information Services.
Scopemed Buttons