Objective: The objective of this study was to observe changes in spermatogenesis testes of albino rats exposed to intraperitoneal lead cetate and to look for the reversibility of these changes after cessation of lead acetate and subsequent oral administration of honey.
Study Design: Experimental animal study.
Place and Duration of Study: National Institute of Health Islamabad from January to June, 2009.
Materials and Methods: Animals were obtained from the animal house of N.I.H and were divided into three groups A, B and C. Group A was subdivided into two groups A-I and A-II. Group B was also subdivided into two sub groups; B-I & B-II. Group C was not subdivided into ubgroups. The animals in groupAwere used as control, while those of groups B and C were treated with lead acetate that was given ntraperitonially in the dose of 4mg/kg body weight, 5 days a week for 6 weeks. The animals in group B-I were sacrificed at the end of six week to observe the toxic changes while animas in group B-II were kept alive for another 6 weeks on normal diet. The animals in group C were given honey in dosage of 10ml/100ml water with normal diet for further 6weeks. These groups (B-II and C) were then sacrificed after 12 weeks to observe the effects of honey on spermatogenesis.
Results: The histological comparison of testes of both groups of animals showed that after six weeks, the width of germinal epithelium and the number of spermatogenic cells had decreased in lead toxic groups as compared to the control rats (p
lead, testes, rat, honey.