Home    Add Your Journal   |    Follow on Twitter   |    Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article



The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica

Gabriel Zamora, Kees Beukelman, Bert van den Berg, Maria Laura Arias, Eduardo Umana, Ingrid Aguilar, Linda Quarles van Ufford, Edwin van den Worm, Natalia Fallas, Rebeca Solorzano.

Abstract
Objective: Medihoney® is considered up to date the only antioxidant therapy specifically approved by the United States Food and Drug Administration (FDA) for wound healing. This is the first study that evaluates the antioxidant capacity and immunomodulatory activity of stingless bee honeys in comparison to Medihoney®. The goal of this study was to evaluate in vitro the potentials of Meliponini honey from Costa Rica as an antioxidant wound dressing agent.
Materials and Methods: A total of fifty-seven honeys belonging to the Tetragonisca angustula (n = 36) and Melipona beecheii (n = 21) species were studied. Following tests were carried out: screening of radical scavenging activity by the 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay, superoxide anion radical scavenging assay, inhibition of xanthine oxidase (XO), total phenolic content by the Folin-Ciocalteu reagent, and the oxygen radical absorbance capacity (ORAC) assay. Furthermore, the effect of honey over reactive oxygen species (ROS) produced by human polymorphonuclear leucocytes (PMNL) was evaluated using luminol and a Cypridina luciferin analog as luminescence probes.
Results: The positive correlation between the total phenolic content and the ORAC value reported herein, suggests that the antioxidant capacities of the stingless bee honeys examined are given in a major grade to their phenolic constituents. According to present results, the in vitro immunomodulatory activities are explained mainly due to the radical scavenging effect and to the XO inhibition. Both mechanisms may play a role in the impairment of the inflammatory process and the promotion of redox homeostasis.
Conclusion: The T.angustula honeys showed antioxidant capacities not statistically different to Medihoney®. In addition, their radical scavenging activity over ROS produced by human PMNLs and inhibition of XO justify their ethnopharmacological use as a wound dressing and further research towards novel wound-healing developments.

Key words: Honey, immunomodulatory, Medihoney, Meliponini


Full text links

Share this Article




ScopeMed Home
Follow ScopeMed on Twitter
Article Tools
Job Opportunities/Service Offers
eJPort Journal Hosting
About ScopeMed
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.
Scopemed Buttons