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Egypt. J. Exp. Biol. (Bot.). 2013; 9(2): 163-170


APPLICATION OF PLACKETT-BURMAN EXPERIMENTAL DESIGN FOR LIPASE PRODUCTION BY A NOVEL ISOLATE OF PSEUDOMONAS FLUORESCENS UTILIZING OLIVE MILL WASTE WATER

Youssef M. Abdel Mawgoud Mohamed E.A. Dawoud.

Abstract
Plackett-Burman design (PBD) was used to efficiently select important medium components affecting the lipase production by a novel Pseudomonas fluorescens isolate utilizing olive mill waste water (OMWW) as the main substrate. Eleven medium components (glucose, olive oil, Tween-80, peptone, yeast extract, sucrose, (NH4)2SO4, NaNO3, Na2HPO4, CaCl2, and MgSO4) were analyzed in twelve experimental trials using PBD. The most significant six components affecting lipase production were found to be olive oil, Tween-80, sucrose, (NH4)2SO4, Na2HPO4, and MgSO4 at p < 0.05. They were found to be contributing positively to the overall lipase production with a maximum production of 0.651 U/ml. Thus, the statistical approach employed in this study allows for rapid identification of important medium parameters affecting the lipase production and the results indicate the efficiency of using PBD for screening processes. However, optimal concentration of the significant components can be determined by further statistical analysis.

Key words: Olive mill waste water, lipase, Pseudomonas fluorescens, Plackett-Burman design



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