Abstract
Aim: To compare the amylase activity in the cervical mucus and serum during estrus in normal and repeat breeder cattle and find out importance in fertility.

Materials and methods: Twenty cyclic crossbred cattle were divided into two groups: normal (n =10) and repeat breeder (n =10). Animals exhibiting spontaneous estrus, were artificially inseminated (AI) twice, as per AM-PM schedule along with simultaneous collection of cervical mucus and blood. Amylase activity was measured by using kits in collected samples.

Results: Cervical amylase activity was significantly (p

Key words: Amylase, Cervical mucus, Estrus, Repeat breeder cattle, Spermatozoa.