Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Research

Vet World. 2010; 3(11): 489-494

Isolation, Serological and Real time PCR diagnosis of Peste Des Petites Ruminants virus in naturally exposed Arabian Gazelle in Saudi Arabia

S. S. Sharawi, M. R. Yousef, A. N. AlHofufy, M. H. AlBlowi.

Oculo-nasal swab and serum samples collected from peste des petits ruminants (PPR)-suspected Arabian Gazelle (Gazella gazella) were tested . For the presence of peste des petite ruminants virus (PPRV) [pest of small ruminants virus] or it’s RNA; Immune-capture enzyme linked immunosorbent assay (Ic-ELIZA), real time reverse transcription-PCR (rRT-PCR) assay using SYBR Green 1 chemistry as well as virus isolation (VI) were done. The serum was examined for the presence of the PPRV anti-bodies by competitive enzyme linked immunosorbent assay (C-ELIZA). The swab samples and harvested inoculated cells were positive by Immune-capture ELIZA and rRT-PCR. While the tested serum was negative for PPRV anti-bodies. The study indicated the scenario of probably virus circulation in these game animal population and prevalence in actual outbreaks situation, which may be kept in mind while deciding the vaccination strategy for the control of disease. For the authors this is the first report of PPRV isolation and detection among Arabian Gazelle in Saudi Arabia

Key words: Arabian Gazelle, enzyme linked immunosorbent assay, Peste des petite ruminants virus, Real time, Sybr Green.

Full text links

Share this Article

Journal of Contemporary Medical Education


ScopeMed Home
Follow ScopeMed on Twitter
Article Tools
eJPort Journal Hosting
About ScopeMed
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.
Scopemed Buttons