Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Article

PAFMJ. 2015; 65(6): 764-767


Dr Jahanzeb ur Rehman, Dr Suhaib Ahmed, Dr Farhat Abbas Bhatti, Dr Nadir Ali.

Objective: To determine the frequency of pyruvate kinase deficiency in neonates presenting with haemolytic anaemia.
Study Design: Cross sectional descriptive study.
Place and Duration of Study: Haematology department, Armed Forces Institute of Pathology, Rawalpindi (AFIP) from Jan 2011 to Jan 2012.
Material and Methods: Study was done in collaboration with neonatology department of Military Hospital. Informed consent from parents of neonates was obtained. Two hundred and twenty five neonates with haemolytic anaemia based on low haemoglobin (5%) and indirect hyper bilirubinaemia (as per CDC nomogram for evaluation of hyper bilirubinaemia in term neonates) were selected. Qualitative pyruvate kinase enzyme assay was done using Bio vision PK assay kit. Estimation of enzyme was based on generation of pyruvate by addition of substrate with change in optical density (OD) of sample in presence of enzyme. Dilution of standard as recommended by manufacturer was made and standard graph was plotted. The OD was measured using wave length of 570 nm at two points (start and at 25 min). Cut off limit of less than 25% activity was considered positive for pyruvate kinase deficiency. Confirmation was done by running in parallel negative and positive controls (provided).
Results: Seven (3.1%Confidence Interval: 3.33) out of 225 patients were found deficient. Among these 4 were male and 3 were female neonates. The age range was between 1 to 3 weeks. The mean age of presentation was 1.89 (0.832) weeks.
Conclusion: We conclude that pyruvate kinase deficiency is not uncommon in our setup and all patients with congenital non-spherocytic haemolytic anaemia where cause cannot be established should be screened for pyruvate kinase deficiency.

Key words: Congenital Non Spherocytic Haemolytic Anaemia, Pyruvate Kinase Deficiency.

Share this Article

American Journal of Diagnostic Imaging


ScopeMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons