For isolation and identification of the antibiotic resistance profile of Staphylococcal infection derived from buffalo (Bubalus bubalis) milk, a total of 100 milk samples have been collected from different villages in Qena governorate, Egypt, these animals were owned by small scale breeding farmers. S. aureus formed 23%, 58% other Staphylococci and other pathogens contained19% of the total number of samples. The S. aureus isolates have been tested for antibiotic susceptibility against 14 different widely used antibiotics, all S. aureus strains were resistant to Nalidixic acid by 100% and the lower percentage of resistance was to Ampicillin by 4.8%. The Multiple antibiotic resistances have been calculated for all S. aureus strains, the highest value was 1 and the least one was 0.143. The Buffaloe is considered one of the most important dairy animals in Egypt especially in the small scale breeding and the presence of S. aureus and other Staphylococci causing a great risk on the productive level of these animals due to mastitis as well as for the human beings, from here comes the importance of the detection of the antibiotic resistance profile.
S. aureus, Antibiotic susceptibility test, Mastitis