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Modified Method for Isolation of Peripheral Blood Neutrophils from Bovines and Humans

Raheela Akhtar, Zafar Iqbal Chaudhary, Yongqun Oliver He.

Cited by (1)

Abstract
A modified method based on the principle of density gradient centrifugation, using lymphocyte separating media was developed for neutrophils isolation from peripheral blood of bovines and humans. The advent of this method with 95% purity, 96% viability, retained morphology and function of neutrophils showed excellent strategy for cell separation.

Key words: Isolation, Neutrophils, protocol.



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