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Veterinary World
Periodical of Veterinary World
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ISSN: 0972-8988 (Print)
ISSN: 2231-0916 (Online)
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Open Access

Original Research



Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats

Sumit Mahajan, Rajesh Agrawal, Mahesh Kumar, Anand Mohan, Nishi Pande.

Cited by (1)

Abstract
Aim: To assess the suitability, sensitivity and specificity of F gene based RT-PCR for detection of PPRV infection in India.

Materials and Methods: A total of 26 blood samples collected from 26 animals suspected for Peste des petits ruminant (PPR) were tested by RT- PCR using F1/F2 primers set and then compared with sandwich-ELISA for detection of PPR virus.

Results: Out of 26 samples tested RT-PCR reported 10 (38.46%) samples as positive as compared to Sandwich-ELISA which reported 11(42.30%) samples as positive for PPR virus. Further analysis of samples revealed that 3 (11.53%) samples which were negative by sandwich-ELISA reported positive with RT-PCR. Whereas 4 (15.38 %) samples shown negative by RT-PCR were positive with sandwich-ELISA. The relative sensitivity and specificity of F-gene based RT-PCR when compared with sandwich-ELISA was 63.60% and 80.00%, respectively. The overall agreement between the two tests was of 73.10% with kappa value 0.442, which suggesting a moderate agreement between the two tests.

Conclusion: A low sensitivity and specificity along with moderate agreement shown by F- gene based RT-PCR when compared with sandwich ELISA suggests that some other highly sensitive and specific primers should be explored for detection of PPR by RT-PCR.

Key words: evaluation, Peste des petits ruminant (PPR), RT- PCR, Sandwich-ELISA.



REFERENCES
1. Singh, R.P. (2011) Control strategies for Peste des petits ruminants in small ruminants of India, Rev. Sci. Tech. 30 (3): 879-887.

[Pubmed]   

2. Van mol, P., Alldinger, S., Baumgartner, W. and Admi, M. (1995) Distemper in wild carnivores: An epidemiological, histological and immunochemical study, Vet. Microbiol., 44: 193-199.


3. Misbah, A., Muhammad, A., Anjum, R., Shamim, S. and Ali, Q. (2009) Prevalence of Peste Des Petits Ruminants Virus (PPRV) in Mardan, Hangu and Kohat District of Pakistan; Comparative Analysis of PPRV Suspected serum samples using Competitive ELISA (cELISA) and Agar Gel Immunodiffusion (AGID), Vet. World, 2(3): 89-92.


4. Banyard, A.C., Parida, S., Batten, C., Oura, C., Kwiatek, O. and Libeau, G. (2010) Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control, J. Gen. Virol., 91: 2885-2897.

[
DOI via Crossref]   
[Pubmed]   

5. Singh, R.K., Balamurugan, V., Bhanuprakash, V., Sen, A., Saravan, P., and Yadav, M.P. (2009) Possible control and eradication of peste des petits ruminants from India: technical aspect, Vet. Ital., 45(3): 449-462.

[
Pubmed]   

6. Chauhan, H.C., Chandel, B.S., Kher, H.N., Dadawala, A.I. and Agrawal, S. M. (2009) Pesti des petits ruminant's virus infection in animals, Vet. World, 2(4):150-155.


7. Shaila, M.S., Purashothaman, V., Bhavsar, D., Venugopal, K. and Venkatesan, R.A. (1989) Peste des petits ruminants of sheep in India, Vet. Rec., 125: 602.

[
Pubmed]   

8. Mahajan, S., Agrawal, R., Kumar, M., Mohan, A. and Pande, N. (2012) Risk of seroconversion to peste des petits ruminants (PPR) and its association with species, sex, age and migration, Small Rum. Res., 104: 195-200.

[
DOI via Crossref]   

9. Bhadyopadhyay, S.K. (2002) The economic appraisal of a PPR control programme in India. In 14th annual conference and national seminar on managememt of viral diseases with emphasis on global trade and WTO regime, Indian virology society, 18-20 January, Hebbal Banglore. Indian virology society, Hisar.


10. Forsyth, M. and Barrett, T. (1995) Detection and differentiation of rinderpest and peste des petits ruminants viruses in diagnostic and experimental samples by polymerase chain reaction using P and F gene-specific primers, Virus Res., 39: 151-163.

[
DOI via Crossref]   

11. George, A. A. (2002) Comparative evaluation of different gene targets for PCR diagnosis of PPR. M.V.Sc. thesis. Deemed University IVRI, Izatnagar, India.


12. Tiwari, A. (2004) Prevalence of Peste des petits ruminants (PPR) virus in small ruminants of Gujarat and its characterization by RT-PCR/ RFLP and SSCP profiles. M.V.Sc. thesis, Anand Agricultural University, Anand, India.


13. Kerur, N. (2005) Assessment of different gene targets for detection of Peste des petits ruminants virus by RT-PCR and sequence analysis of F and N gene segments. M.V.Sc. thesis, Anand Agricultural University, Anand, India.


14. Kerur, N., Jhala, M.K. and Joshi C.G. (2008) Genetic characterization of Indian peste des petits ruminants virus (PPRV) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments, Res. Vet. Sci., 85: 176–183.1

[
DOI via Crossref]   
[Pubmed]   

15. 2-way Contingency Table Analysis., In http://statpages.org/ctab2x2.html. Retrieved on 20/07/10.


16. Chauhan, H.C., Kher, H.N., Dadawala, A.I., Kumar, P., Sen, A., Chandel, B.S. (2012) Detection of Peste des petits ruminants virus in sheep and goats, Indian Vet. J., 89(8): 60-62.

This Article Cited By the following articles

Advances in Diagnosis of Respiratory Diseases of Small Ruminants
Veterinary Medicine International 2014; 2014(): 1.

1
 

How to Cite this Article
Pubmed Style

Mahajan S, Agrawal R, Kumar M, Mohan A, Pande N. Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats. Vet World. 2013; 6(6): 288-290. doi:10.5455/vetworld.2013.288-290



Web Style

Mahajan S, Agrawal R, Kumar M, Mohan A, Pande N. Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats. www.scopemed.org/?mno=25029 [Access: September 20, 2014]. doi:10.5455/vetworld.2013.288-290



AMA (American Medical Association) Style

Mahajan S, Agrawal R, Kumar M, Mohan A, Pande N. Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats. Vet World. 2013; 6(6): 288-290. doi:10.5455/vetworld.2013.288-290



Vancouver/ICMJE Style

Mahajan S, Agrawal R, Kumar M, Mohan A, Pande N. Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats. Vet World. (2013), [cited September 20, 2014]; 6(6): 288-290. doi:10.5455/vetworld.2013.288-290



Harvard Style

Mahajan, S., Agrawal, R., Kumar, M., Mohan, A. & Pande, N. (2013) Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats. Vet World, 6 (6), 288-290. doi:10.5455/vetworld.2013.288-290



Turabian Style

Mahajan, Sumit, Rajesh Agrawal, Mahesh Kumar, Anand Mohan, and Nishi Pande. 2013. Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats. Veterinary World, 6 (6), 288-290. doi:10.5455/vetworld.2013.288-290



Chicago Style

Mahajan, Sumit, Rajesh Agrawal, Mahesh Kumar, Anand Mohan, and Nishi Pande. "Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats." Veterinary World 6 (2013), 288-290. doi:10.5455/vetworld.2013.288-290



MLA (The Modern Language Association) Style

Mahajan, Sumit, Rajesh Agrawal, Mahesh Kumar, Anand Mohan, and Nishi Pande. "Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats." Veterinary World 6.6 (2013), 288-290. Print. doi:10.5455/vetworld.2013.288-290



APA (American Psychological Association) Style

Mahajan, S., Agrawal, R., Kumar, M., Mohan, A. & Pande, N. (2013) Comparative evaluation of RT-PCR with sandwich-ELISA for detection of Peste des petits ruminant in sheep and goats. Veterinary World, 6 (6), 288-290. doi:10.5455/vetworld.2013.288-290



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