Staphylococcus aureus (MRSA and MSSA) and Escherichia coli strains were isolated from the Clinical specimens such as pus, wound, urine, ear swabs and blood collected from the Health care centers at Lucknow city. Reference strains of Staphylococcus aureus and Escherichia coli were procured from IMTECH Chandigarh. All strains were tested and detected as multi-drug resistant against penicillin, ampicillin, cefpdoxime, sulphadiazine, nalidixic acid, erythromycin and amoxicillin. Antibacterial activity of Syzigium cumini leaf extracts in various solvents was also determined. The efficacy of the extracts was found higher in petroleum ether and ethanolic solvents with the inhibition zone 8-24 mm than all other solvent extracts tested. Extracts of Syzygium cumini exhibited prominent activity against both the drug resistant strains of S. aureus and E. coli and their activity is quite comparable with the antibiotics such as penicillin, ciprofloxacin, erythromycin, tetracycline, kanamycin, ofloxacin and amoxicillin etc. Minimum inhibitory and minimum bactericidal concentrations (MIC and MBC) of Syzigium cumini leaf extracts were also determined among the strains. MIC and MBC values varied from 1.56 to 25 mg/ml and 1.56 to 50 mg/ml among the S. aureus and E. coli strains respectively. The combination of ampicillin with plant extract exhibited a significant synergistic effect on growth of multidrug resistant strains of both genus tested. Our study thus suggests the use of this medicinal plant in the treatment of various diseases caused by drug resistant species of S. aureus and E. coli.
Keywords: Syzygium cumini, antibacterial activity, Clinical Specimens, MDR strains, synergistic effect.