In the clinical isolates of Gram negative Bacilli, Metallo Beta lactamases (MBL) production had been seen. Isolates resistant to Imipenem were screened for MBL production by EDS (EDTA Disc Synergy) test, Modified Hodge test and MIC Reduction test. There was comparison among these methods. Rapid detection of MBL producing Gram negative bacilli is necessary to prevent their dissemination and also it provides therapeutic guidance.
MBL (Metallo Beta Lactamase), MIC (Minimal Inhibitory Concentration), EDS (EDTA Disc Synergy).