IJHSR. 2016; 6(12): 210-218
Biochemical Variations and FTIR-ATR Spectral Studies on Renal Disease Induced by Gentamicin in Male Wistar Rats. Sairakhatheeja, Prabhakaran, A.R., Krishna Mohan, S, Selvanathan, P, Safiullah, A.
Renal Disease (RD) is a major health burden, which has received increased attention in recent times and has thus become one major focus of intensive research. Commonly used animal models for RD are bedeviled by methodologically induced complexities, which make the procedures not only laborious but also make interpretation of results less explicit. We have therefore characterized a simple and reproducible method for inducing renal failure in rats; in which the pathological parameters better reflect the usual findings in clinical situations. This approach has methodological and experimental advantages with respect to commonly used procedures for inducing RD and studying biochemical variations experimental rats. FTIR-ATR spectroscopy has significant advantages composed to many other methods for the characterization of biochemical molecules because it relies on the characteristic absorbance of corresponding molecular vibration in the sample functional group of chemical compounds such as carbohydrates, ester, albumin, proteins, as well as inter atom chemical bonds. This study attempts to evaluate the spectral difference between healthy and rat induced renal disease To achieve this male wistar rat weighing 180 +15 gms were chosen for control and the experimental rats followed by injection of gentamicin subcutaneously for 21 days as per standardized protocol. The renal failure was assessed by elevated blood urea and serum creatinine and results showed significant biochemical variations in blood serum. At the end, control and experimental animals’ blood serum were analysed for FTIR-ATR spectral evaluation and quantification of biomolecules. The simplicity and reproducibility of this model, coupled with a better correlation with the known features of RD makes it a useful rat model not only for research purposes but also for testing of therapeutic maneuvers commonly used in the clinical Diagnosis. The significance of FTIR -ATR spectroscopy in analyzing biochemical variations in blood serum in experimental animals discussed statistically.
FTIR-ATR, Gentamicin Renal Disease, Spectral Analysis.