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Veterinary World
Periodical of Veterinary World
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ISSN: 0972-8988 (Print)
ISSN: 2231-0916 (Online)
Language: [ English ]  
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Open Access

Original Research



Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs

Vinay G. Joshi, Arvind Kumar Singh, Kantaraja Chindera, Manish V. Bais, Ashok Kumar Tiwari, Satish Kumar.

A B S T R A C TR E F E R E N C E SCited by (1) 

Abstract
Aim: This study was designed to develop peptide analogs of Infectious Bursal Disease (IBD) virus VP5 protein segment
having cell penetrating ability to improve their interaction with cargo molecule (Nucleic acid) without affecting the backbone conformation.

Materials and Methods: IBDV VP5 protein segment designated as RATH peptide were synthesized using solid phase
peptide synthesis and their solution conformation was elucidated using CD spectroscopy in polar (water) and apolar (TFE) solvents. Cell penetrating ability of RATH-CONH2 was observed using FITC labeled peptide internalization in to HeLa cells under fluorescent microscopy. The efficacy of RATH analog interactions with nucleic acids was evaluated using FITC labeled oligonucleotides by fluorescence spectroscopy and plasmid constructs in gel retardation assay.
Results: CD spectra of RATH analogs in water and apolar trifluroethanol (TFE) helped to compare their secondary structures which were almost similar with dominant beta conformations suggesting successful induction of positive charge in the analogs without affecting back bone conformation of CPP designed. Cell penetrating ability of RATH CONH2 in HeLa cell was more than 90%. The fluorescence spectroscopy and plasmid constructs in gel retardation assay demonstrated successful interaction of amide analogs with nucleic acid.

Conclusion: Intentional changes made in IBDV derived peptide RATH COOH to RATH CONH2 did not showed major
changes in backbone conformation and such modifications may help to improve the cationic charge in most CPPs to interact with nucleic acid.

Key words: IBDV, conformationa analysis, gel retardation assay, RATH, VP5 protein


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This Article Cited By the following articles

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Indian J. Virol. 2013; (): .

1
 

How to Cite this Article
Pubmed Style

Joshi VG, Singh AK, Chindera K, Bais MV, Tiwari AK, Kumar S. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Vet World. 2013; 6(6): 307-312. doi:10.5455/vetworld.2013.307-312



Web Style

Joshi VG, Singh AK, Chindera K, Bais MV, Tiwari AK, Kumar S. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. www.scopemed.org/?mno=26756 [Access: April 23, 2014]. doi:10.5455/vetworld.2013.307-312



AMA (American Medical Association) Style

Joshi VG, Singh AK, Chindera K, Bais MV, Tiwari AK, Kumar S. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Vet World. 2013; 6(6): 307-312. doi:10.5455/vetworld.2013.307-312



Vancouver/ICMJE Style

Joshi VG, Singh AK, Chindera K, Bais MV, Tiwari AK, Kumar S. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Vet World. (2013), [cited April 23, 2014]; 6(6): 307-312. doi:10.5455/vetworld.2013.307-312



Harvard Style

Joshi, V. G., Singh, A. K., Chindera, K., Bais, M. V., Tiwari, A. K. & Kumar, S. (2013) Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Vet World, 6 (6), 307-312. doi:10.5455/vetworld.2013.307-312



Turabian Style

Joshi, Vinay G., Arvind Kumar Singh, Kantaraja Chindera, Manish V. Bais, Ashok Kumar Tiwari, and Satish Kumar. 2013. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Veterinary World, 6 (6), 307-312. doi:10.5455/vetworld.2013.307-312



Chicago Style

Joshi, Vinay G., Arvind Kumar Singh, Kantaraja Chindera, Manish V. Bais, Ashok Kumar Tiwari, and Satish Kumar. "Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs." Veterinary World 6 (2013), 307-312. doi:10.5455/vetworld.2013.307-312



MLA (The Modern Language Association) Style

Joshi, Vinay G., Arvind Kumar Singh, Kantaraja Chindera, Manish V. Bais, Ashok Kumar Tiwari, and Satish Kumar. "Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs." Veterinary World 6.6 (2013), 307-312. Print. doi:10.5455/vetworld.2013.307-312



APA (American Psychological Association) Style

Joshi, V. G., Singh, A. K., Chindera, K., Bais, M. V., Tiwari, A. K. & Kumar, S. (2013) Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Veterinary World, 6 (6), 307-312. doi:10.5455/vetworld.2013.307-312







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