Journal Management System 		ScopeMed is an
Online Journal/Article Management system
that can enable all of the operations of
the editorial functions of a journal.
Home  |   Add your Journal  |   Properties of ScopeMed  |   Video Tutorials  |   Journal List
Author Login | Reviewer Login | Editor Login
Search  


Veterinary World
Periodical of Veterinary World
Journal Home Page		ISSN: 0972-8988 (Print)
ISSN: 2231-0916 (Online)
Language: [ English ]  

  » Volume 6, Issue 8
      pp. 461-524
  » Volume 6, Issue 7
      pp. 370-460
  » Volume 6, Issue 6
      pp. 285-369
  » Volume 6, Issue 5
      pp. 239-284
  » Volume 6, Issue 4
      pp. 180-238
  » Volume 6, Issue 3
      pp. 122-179
  » Volume 6, Issue 2
      pp. 64-121
  » Volume 6, Issue 1
      pp. 1-63

 

Open Access

Original Research



Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs

Vinay G. Joshi, Arvind Kumar Singh, Kantaraja Chindera, Manish V. Bais, Ashok Kumar Tiwari, Satish Kumar.

A B S T R A C TR E F E R E N C E S 

Abstract
Aim: This study was designed to develop peptide analogs of Infectious Bursal Disease (IBD) virus VP5 protein segment
having cell penetrating ability to improve their interaction with cargo molecule (Nucleic acid) without affecting the backbone conformation.

Materials and Methods: IBDV VP5 protein segment designated as RATH peptide were synthesized using solid phase
peptide synthesis and their solution conformation was elucidated using CD spectroscopy in polar (water) and apolar (TFE) solvents. Cell penetrating ability of RATH-CONH2 was observed using FITC labeled peptide internalization in to HeLa cells under fluorescent microscopy. The efficacy of RATH analog interactions with nucleic acids was evaluated using FITC labeled oligonucleotides by fluorescence spectroscopy and plasmid constructs in gel retardation assay.
Results: CD spectra of RATH analogs in water and apolar trifluroethanol (TFE) helped to compare their secondary structures which were almost similar with dominant beta conformations suggesting successful induction of positive charge in the analogs without affecting back bone conformation of CPP designed. Cell penetrating ability of RATH CONH2 in HeLa cell was more than 90%. The fluorescence spectroscopy and plasmid constructs in gel retardation assay demonstrated successful interaction of amide analogs with nucleic acid.

Conclusion: Intentional changes made in IBDV derived peptide RATH COOH to RATH CONH2 did not showed major
changes in backbone conformation and such modifications may help to improve the cationic charge in most CPPs to interact with nucleic acid.

Key words: IBDV, conformationa analysis, gel retardation assay, RATH, VP5 protein


ARTICLE TOOLS
Abstract
PDF Fulltext
Email this Article
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Vinay G. Joshi
Articles by Arvind Kumar Singh
Articles by Kantaraja Chindera
Articles by Manish V. Bais
Articles by Ashok Kumar Tiwari
Articles by Satish Kumar
on IndexScholar Fulltext
on Google
on Google Scholar
Article Statistics
 Viewed: 252
Downloaded: 114
(Last 7 Days: 3)
Cited: 0
Emailed: 0

REFERENCES
1. Whitehead K.A., Langer R. and Anderson D.G. (2009) Knocking down barriers: advances in siRNA delivery, Nat. Rev. Drug Dis. 8: 129-138.

[DOI via Crossref]   
[Pubmed]   

2. Spagnou S., Miller A.D. and Keller M. (2004) Lipidic Carriers of siRNA: Differences in the Formulation, Cellular Uptake, and Delivery with Plasmid DNA, Biochem., 43: 13348-13356.

[DOI via Crossref]   
[Pubmed]   

3. Storrie H. and Mooney D.J. (2006) Sustained delivery of plasmid DNA from polymeric scaffolds for tissue engineering, Adv. Drug Deliv. Rev. 58: 500-514.

[DOI via Crossref]   
[Pubmed]   

4. Noh S.M., Kim W., Kim S.J., Kim J.M., Kwang-Hyun Baek K.H. and Oh Y.K. (2007) Enhanced cellular delivery and transfection efficiency of plasmid DNA using positively charged biocompatible colloidal gold nanoparticles, Biochim Biophys Acta. 1770: 747-752.

[DOI via Crossref]   
[Pubmed]   

5. Xua Z.P., Zeng Q.H., Lu G.Q. and Yu A.B. (2006) Inorganic nanoparticles as carriers for efficient cellular delivery, Chem. Engg. Sci. 61:1027-1040.

[DOI via Crossref]   

6. Waehler, R., Russell, S.J. and Curiel, D.T. (2007) Engineering targeted viral vectors for gene therapy, Nat. Rev. Genet. 8: 573-587.

[DOI via Crossref]   
[Pubmed]   

7. Kiefer K., Clement J., Garidel P. and Peschka-Suss R. (2004) Transfection efficiency and cytotoxicity of non viral gene transfer reagents in human smooth muscle and endothelial cells, Pharm. Res. 21:1009-1017.

[DOI via Crossref]   
[Pubmed]   

8. Torchilin V.P, Rammohan R, Weissig V and Levchenko T.S. (2001) TAT peptide on the surface of liposomes affords their efficient intracellular delivery even at low temperature and in the presence of metabolic inhibitors, Proc. Natl Acad. Sci., USA. 98: 8786-8791.

[DOI via Crossref]   
[Pubmed]    [PMC Free Fulltext]   

9. Dietz, G.P. and Bahr, M. (2004) Delivery of bioactive molecules into the cell: the Trojan horse approach, Mol Cell Neurosci. 27:85-131.

[DOI via Crossref]   
[Pubmed]   

10. Richard J.P., Melikov K., Vives E., Ramos C., Verbeure B., Gait M.J.,Chernomordik L.V. and Lebleu B. (2003) Cell penetrating peptides: A reevaluation of the mechanism of cellular uptake, J. Biol. Chem. 278: 585-590.

[DOI via Crossref]   
[Pubmed]   

11. Jacobson K., Mouritsen O. G. and Anderson R.G. (2007) Lipid rafts: at a crossroad between cell biology and physics. Nat. Cell. Biol. 9: 7-14.

[DOI via Crossref]   
[Pubmed]   

12. Johnson L.N., Cashman S.M. and Singh R. K., (2008) Cell- penetrating peptide for enhanced delivery of nucleic acids and drugs to ocular tissues including retina and cornea, Mol. Ther. 16: 107–114.

[DOI via Crossref]   
[Pubmed]   

13. Williams J.A., Carnes A.E. and Hodgson C.P. (2009) Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production, Biotechnol. Adv. 27: 353-370.

[DOI via Crossref]   
[Pubmed]    [PMC Free Fulltext]   

14. Langel, U. (2007) Ed. Handbook of Cell-Penetrating Peptides, CRC Press: Boca Raton, FL, USA.

15. Oehlke J., Scheller A., Wiesner B., Krause E., Beyermann M., Klauschenz E., Melzig M. and Bienert M.(1998) Cellular uptake of an alpha-helical amphipathic model peptide with te potential to deliver polar compounds into the cell interior non endocytically, Biochim. Biophys. Acta. 1414: 127–139.

[DOI via Crossref]   

16. Morris M.C., Chaloin L., Mery J.,Heitz F. and Divita G.(1999) A novel potent strategy for gene delivery using a single peptide vector as a carrier, Nucleic Acids Res. 27: 3510-3517.

[DOI via Crossref]   
[Pubmed]    [PMC Free Fulltext]   

17. Bais M.V., Kumar S., Tiwari A.K., Kataria R.S., Nagaleekar V.K., Shrivastava, S. and Chindera K. (2008) Novel Rath peptide for intracellular delivery of protein and nucleic acids, Biochem. Biophys. Res. Commun. 370: 27–32.

[DOI via Crossref]   
[Pubmed]   

18. Anderson D.C., Nichols E., Manger R., Woodle D., Barry M. and Fritzberg A.R.(1993) Tumor cell retention of antibody Fab fragments is enhanced by an attached HIV TAT protein-derived peptide, Biochem. Biophys. Res. Commun, 194: 876–884.

[DOI via Crossref]   
[Pubmed]   

19. Vives E., Richard J.P., Rispal C. and Lebleu B. (2003) TAT peptide internalization: seeking the mechanism of entry, Curr. Protein Pept. Sci. 4: 125–132.

[DOI via Crossref]   
[Pubmed]   

20. Fawell S., Seery J., Daikh Y., Moore C., Chen L.L., Pepinsky B. and Barsoum J. (1994) Tat-mediated delivery of heterologous proteins into cells. Proc. Natl. Acad. Sci. USA, 91: 664-668.

[DOI via Crossref]   
[Pubmed]    [PMC Free Fulltext]   

21. Satish Kumar., Bais M.V., Kataria R.S. and Yadav M.P. (2008) An artificially synthesized peptide. International PCT WIPO Publication No. WO/2008/041047.

How to Cite this Article
Pubmed Style

Joshi VG, Singh AK, Chindera K, Bais MV, Tiwari AK, Kumar S. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Vet World. 2013; 6(6): 307-312. doi:10.5455/vetworld.2013.307-312


Web Style

Joshi VG, Singh AK, Chindera K, Bais MV, Tiwari AK, Kumar S. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. www.scopemed.org/?mno=26756 [Access: May 23, 2013]. doi:10.5455/vetworld.2013.307-312


AMA (American Medical Association) Style

Joshi VG, Singh AK, Chindera K, Bais MV, Tiwari AK, Kumar S. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Vet World. 2013; 6(6): 307-312. doi:10.5455/vetworld.2013.307-312


Vancouver/ICMJE Style

Joshi VG, Singh AK, Chindera K, Bais MV, Tiwari AK, Kumar S. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Vet World. (2013), [cited May 23, 2013]; 6(6): 307-312. doi:10.5455/vetworld.2013.307-312


Harvard Style

Joshi, V. G., Singh, A. K., Chindera, K., Bais, M. V., Tiwari, A. K. & Kumar, S. (2013) Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Vet World, 6 (6), 307-312. doi:10.5455/vetworld.2013.307-312


Turabian Style

Joshi, Vinay G., Arvind Kumar Singh, Kantaraja Chindera, Manish V. Bais, Ashok Kumar Tiwari, and Satish Kumar. 2013. Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Veterinary World, 6 (6), 307-312. doi:10.5455/vetworld.2013.307-312


Chicago Style

Joshi, Vinay G., Arvind Kumar Singh, Kantaraja Chindera, Manish V. Bais, Ashok Kumar Tiwari, and Satish Kumar. "Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs." Veterinary World 6 (2013), 307-312. doi:10.5455/vetworld.2013.307-312


MLA (The Modern Language Association) Style

Joshi, Vinay G., Arvind Kumar Singh, Kantaraja Chindera, Manish V. Bais, Ashok Kumar Tiwari, and Satish Kumar. "Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs." Veterinary World 6.6 (2013), 307-312. Print. doi:10.5455/vetworld.2013.307-312


APA (American Psychological Association) Style

Joshi, V. G., Singh, A. K., Chindera, K., Bais, M. V., Tiwari, A. K. & Kumar, S. (2013) Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs. Veterinary World, 6 (6), 307-312. doi:10.5455/vetworld.2013.307-312


Submit your manuscriptRegisterAbstracting and IndexingInstructions for AuthorsSign up for Article Alerts

» Last Downloads

Please Wait...



Total Download: 235.489
(for Veterinary World)
Total Submission during last 24 hours: 148

» For Authors



Become Reviewer

» Login Area

Follow ScopeMed on Twitter

ScopeMed Home | About ScopeMed | Contact Us | Terms & Conditions | Privacy Policy
The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Service of SAGE Publishing for Scientific Publications. Copyright © ScopeMed® International Medical Journal Management and Indexing System.