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IJCRR. 2012; 4(19): 62-68


GENETIC DIVERSITY IN FIVE CICER GENOTYPES USING OPM-05 PRIMER

Jahangir Ahmad, Vaseem Raja, Rajdeep Kudesia.

Abstract
Five genotypes of Cicer (Dhawal, Ujjwal, Shubhra, DCP-92-3 and IPC-04-20) were analyzed to estimate genetic diversity by using OPM-05 primer. A total of 21 bands were detected, all of them were polymorphic. Similarity index calculated was found highest between Ujjwal and IPC-04-20 (37.5%) and lowest between Shubhra and IPC-04-20 (22.22%). Dice’s similarity coefficient ranged from 0.00000 to 0.25000. A dendrogram constructed based on UPGMA revealed two clusters. Cluster 1 consists of three accessions-S1, S3 and S5 while as cluster 2 consists of two accessions- S2 and S4. It is evident from the dendrogram that accession DCP-92-3 and IPC-04-20 are genetically dissimilar; hence it is recommended that these should be used for future breeding programs to create higher amount of genetic variability in chickpea.

Key words: Genetic diversity, Cicer, RAPD, UPGMA dendrogram



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