Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Article

JVS. 2018; 3(1): 10-19
doi: 282147

Genetic characterization of rabbit hemorrhagic disease virus from naturally-infected rabbits in Sharkia governorate, Egypt

Naglaa F.S. Awad, Gamelat K.F. Kotb.

Since the first introduction of rabbit hemorrhagic disease virus (RHDV) in 1991, Egyptian authorities started a national vaccination program against the disease. However, RHDV clinical cases are still being reported. This study aimed to investigate genetic characterization of recent RHDV isolates from naturally-infected rabbits in Sharkia governorate, Egypt in 2015/2016. A total of 24 clinical samples were investigated by real-time reverse transcription PCR (RRT-PCR). Only two samples were positive for partial C-terminal VP60 amplification. The first positive sample was collected from non-vaccinated young rabbit (45-day old) and designed here as SHAH2015, whereas the second sample was collected from non-vaccinated adult dam (nine-month old) and designed as SHMK2016. Hemagglutination analysis using human red blood cells type O revealed that both isolates were non-hemagglutinating. Sequence analysis revealed that both isolates shared amino acid identity of 98.57 % and 95.71 % with each other and with commonly used vaccinal strain (RHDV-Giza 2006), respectively. Phylogenetic analysis revealed that both isolates clustered with the vaccine strain and isolate UT01 within genotype 6 RHDVa. Molecular analysis of antigenic regions of VP60 (part of E and F regions) revealed neither detection of recombination points nor sites under positive selection. Our results indicate that non-hemagglutinating RHDVa G6 viruses are currently circulating in Sharkia with the ability to infect both young and adult rabbits that may be due to inadequate application of vaccine and not due to vaccine mismatch. We recommend broad application of currently applied vaccine should include both young and adult rabbits.

Key words: RHDV, VP60, vaccination, recombination, RRT-PCR, Egypt

Share this Article

Journal of Contemporary Medical Education


ScopeMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons