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Original Research

RMJ. 2013; 38(4): 354-357


Accuracy of Accutrend GCT meter for the measurement of blood cholesterol levels

Abdul Rehman Arshad.

Abstract
Objective: This study was done to assess the accuracy of Accutrend GCT meter in determining blood cholesterol levels in our setup, with an aim to recommend its use in clinical practice
Methods: This cross sectional study was conducted on 251 healthy volunteers from April to May 2013. Capillary whole blood samples were obtained using sterile lancets and cholesterol levels were measured using Accutrend GCT meter (Roche Diagnostics, Mannheim, Germany) using the standard technique. Three ml whole blood was also collected in plain tubes by the same operator without applying a tourniquet. Serum cholesterol levels were determined using Merck Microlab 300 Automated Clinical Chemistry Analyser the same day. The laboratory technician performing this test was blinded to results obtained with Accutrend GCT meter. The seven patients with readings beyond the analytic range of the Accutrend GCT meter were excluded, meaning that data analysis was performed on 244 results only.
Results: Results of 182 males and 62 females having a mean age of 38.54 15.25 years were analysed. There was a significant difference in mean cholesterol levels measured by Accutrend GCT and Microlab-300 (4.73 0.74mmol/l and 4.67 0.86 mmol/l; p=0.021), producing a measurement bias of 1.43% with Accutrend GCT meter. A strong positive correlation was found between the results of the two techniques (r=0.852 ,p

Key words: Cholesterol, Lipids, Bias, Point-of-Care Systems, Point-of-Care Technology



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