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IJAVMS. 2011; 5(1): 43-52

Exploitation of Different Agro-residues for Acid protease Production by Rhizopus sp. in Koji Fermentation

Muhammad Irfan, Abdul Rauf, Quratualain Syed, Muhammad Nadeem, Shahjahan Baig.

Two different strains of Rhizopus sp. i.e. R. oligosporus -M30 and R. arrhizus -M26 were employed for the production of acid protease using different agro-residues such as rice husk, rice bran, wheat bran, defatted soybean meal and sunflower meal as a sole carbon source. R. oligosporus -M30 gave maximum enzyme level with soybean meal (15.6 U/gds) while R. arrhizus -M26 with sunflower meal (13.6 U/gds) respectively. The optimal conditions for the enzyme production by R .oligosporus -M30 were initial pH of 5.5, temperature 30 oC with 10% of inoculum size for 72hr of fermentation period. When R. arrhizus -M26 was cultivated that exhibit the best enzyme production with the fermentation period of 72h, initial pH 5.0, temperature 33 oC with 5% of inoculum size

Key words: Agro-residues, acid protease, Rhizopus sp., solid state fermentation

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