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EDTA chelation therapy in the treatment of toxic metals exposure

Nina Mikirova, Jiseph Casciari, Ronald Hunninghake, Neil Riordan.

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BACKGROUND: Metal induced toxicity with wide range of physiological, biochemical and behavioral dysfunctions was reported in many studies. The chelation has been used for treatment to toxic metals’ exposure for many years. In our current clinical study, we compared different chelation protocols and two forms of EDTA (sodium calcium edetate and sodium edetate) in treatments of toxic metal exposure.
METHODS: A 24 h urine samples were collected from each subject before and after treatment by Ca-EDTA or Na-EDTA. The levels of toxic and essential metals were measured by Atomic Absorption Spectrometer (AAS) with graphite furnace and by Inductively Coupled Plasma Atomic Emission Spectrometer (ICP-AES). The cellular levels of ATP were determined by ATP-bioluminescence assay. Mitochondrial potential was measured by fluorometer and flow-cytometer after cells’ staining by mitochondrial dye.
RESULTS: Data from over 600 patients with a variety of complaints, but not acute toxic mineral exposure, given chelation therapy with sodium EDTA or calcium EDTA, were analyzed. Ca-EDTA and Na-EDTA at intravenous infusions of 3 g per treatment were equally effective in removing lead from the body, while Ca-EDTA was more effective in aluminum removal. The removal of lead was dose dependent but non-linear. Chelation by different doses of Na-EDTA (3 g and 1 g) resulted in mean difference in lead urine less than 50%. In addition, deficiencies in essential minerals correlated with greater pre-treatment lead and aluminum levels. The intrinsic toxicity of EDTA on cells was investigated. EDTA concentrations above 600 uM reduced cellular energy metabolism.
CONCLUSION: Based on our data, we proposed that low dosages of chelating agents might be preferential for patients with no–occupational exposure, as the benefit of chelation is not linear with dosage, the risk of exposure to antidotes is increasing with increased dosages, and excretion of essential metals significantly increases with increased antidote dosage.

Key words: NADH, chelation, lead, essential metals

Toksik metallere maruz kalm tedavisinde EDTA elasyon terapisi

GR: Birok almada, metal zehirlenmelerine fizyolojik, biyokimyasal ve davransal fonksiyon bozukluklarnn elik ettii rapor edilmiitir. Toksik metallere maruz kalm tedavisi iin yllardr elasyon kullanlmaktadr. imdiki klinik almamzda, toksik metallere maruz kalmn tedavisinde, farkl elasyon protokollerini ve iki EDTA formunu (sodyum kalsiyum EDTA ve sodyum EDTA) karlatrdk.
YNTEM: Ca-EDTA veya Na-EDTA ile tedavi ncesinde ve sonrasnda, her denekten 24 saatlik idrar rnekleri topland. Toksik ve esansiyel metallerin dzeyleri, grafit frnl atomik absorbsiyon spektrometresi (AAS) ve indktif olarak eletirilmi plazma atomik emisyon spektrometresi (ICP-AES) ile lld. Hcresel ATP dzeyleri, ATP-biyolminesans analizi ile saptand. Mitokondriyal potansiyel, hcrelerin mitokondri boyas ile boyanmasndan sonra florimetre ve ak sitometresi ile lld.
BULGULAR: eitli ikayetleri olan fakat akut toksik mineral maruz kalm olmayan, Na-EDTA ve Ca-EDTA ile elasyon tedavisi alan 600’n zerindeki hastadan elde edilen veriler deerlendirildi. Ca-EDTA ve Na-EDTA’nn tedavi bana 3 g intravenz infzyonu, kurunun vcuttan uzaklatrlmasnda eit derecede etkinken; Ca-EDTA, alminyumun uzaklatrlmasnda daha etkindi. Kurunun uzaklatrlmas, doz bamlyd; fakat, dorusal deildi. Farkl Na-EDTA dozlar (3 g ve 1 g) ile elasyon, idrar kurununda %50’den daha az ortalama farkllkla sonuland. Ayrca, esansiyel minerallerdeki eksiklikler, tedavi ncesi daha fazla kurun ve alminyum dzeyleri ile orantlyd. Hcreler zerine EDTA’nn intrensek toksisitesi aratrld. EDTA, 600 μM zerindeki konsantrasyonlarda hcresel enerji metabolizmasn azaltt.
SONU: Verilere gre; elasyonun faydas dozla orantl olmadndan, antidotlara maruziyet riski yksek dozlarda arttndan ve esansiyel metallerin atlm yksek antidot dozlar ile arttndan dolay, elasyon ajanlarnn dk dozlar mesleki olmayan maruz kalmlar iin ncelikli olabilir.

Anahtar Kelimeler: NADH, elasyon, kurun, esansiyel metaller

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