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Veterinary World
Periodical of Veterinary World
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ISSN: 0972-8988 (Print)
ISSN: 2231-0916 (Online)
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Open Access

Review Article



Molecular diagnosis of Haemorrhagic Septicaemia - A Review

Ranjan Rajeev, S. K. Panda, A. P. Acharya, A. P. Singh, M. K. Gupta.

Abstract
Pasteurella multocida is associated with hemorrhagic septicaemia in cattle and buffaloes, pneumonic pasteurellosis in sheep and goats, fowl cholera in poultry, atrophic rhinitis in pigs and snuffles in rabbits. Haemorrhagic septicaemia is caused by Pasteurella multocida type B:2, B:2,5 and B:5 in Asian countries and type E:2 in African countries. Pasteurella multocida have five types of capsular serotype i.e. type A, B, D, E and F. Diagnosis of the disease is mainly based on the clinical sign and symptom, post mortem findings. Confirmatory diagnosis is done by isolation and identification of causative agent. A variety of laboratory diagnostic techniques have been developed over the years for pasteurellosis and used routinely in the laboratory. Among these techniques molecular techniques of diagnosis is most important. This technique not only gives diagnosis but it also provides information regarding capsular type of Pasteurella multocida. Techniques which are used for molecular diagnosis of haemorrhagic septicaemia are PCR based diagnosis, Restriction endonuclease analysis (REA), Ribotyping, Colony hybridization assay, Filled alternation gel electrophoresis (FAGE), Detection of Pasteurella multocida by Real Time PCR. Among these techniques real time PCR is most sensitive and specific.

Key words: Haemorrhagic septicaemia, Molecular diagnosis, Laboratory diagnostic technique, Real time PCR.



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How to Cite this Article
Pubmed Style

Rajeev R, Panda SK, Acharya AP, Singh AP, Gupta MK. Molecular diagnosis of Haemorrhagic Septicaemia - A Review. Vet World. 2011; 4(4): 189-192. doi:10.5455/vetworld.2011.189-192



Web Style

Rajeev R, Panda SK, Acharya AP, Singh AP, Gupta MK. Molecular diagnosis of Haemorrhagic Septicaemia - A Review. www.scopemed.org/?mno=6411 [Access: September 21, 2014]. doi:10.5455/vetworld.2011.189-192



AMA (American Medical Association) Style

Rajeev R, Panda SK, Acharya AP, Singh AP, Gupta MK. Molecular diagnosis of Haemorrhagic Septicaemia - A Review. Vet World. 2011; 4(4): 189-192. doi:10.5455/vetworld.2011.189-192



Vancouver/ICMJE Style

Rajeev R, Panda SK, Acharya AP, Singh AP, Gupta MK. Molecular diagnosis of Haemorrhagic Septicaemia - A Review. Vet World. (2011), [cited September 21, 2014]; 4(4): 189-192. doi:10.5455/vetworld.2011.189-192



Harvard Style

Rajeev, R., Panda, S. K., Acharya, A. P., Singh, A. P. & Gupta, M. K. (2011) Molecular diagnosis of Haemorrhagic Septicaemia - A Review. Vet World, 4 (4), 189-192. doi:10.5455/vetworld.2011.189-192



Turabian Style

Rajeev, Ranjan, S. K. Panda, A. P. Acharya, A. P. Singh, and M. K. Gupta. 2011. Molecular diagnosis of Haemorrhagic Septicaemia - A Review. Veterinary World, 4 (4), 189-192. doi:10.5455/vetworld.2011.189-192



Chicago Style

Rajeev, Ranjan, S. K. Panda, A. P. Acharya, A. P. Singh, and M. K. Gupta. "Molecular diagnosis of Haemorrhagic Septicaemia - A Review." Veterinary World 4 (2011), 189-192. doi:10.5455/vetworld.2011.189-192



MLA (The Modern Language Association) Style

Rajeev, Ranjan, S. K. Panda, A. P. Acharya, A. P. Singh, and M. K. Gupta. "Molecular diagnosis of Haemorrhagic Septicaemia - A Review." Veterinary World 4.4 (2011), 189-192. Print. doi:10.5455/vetworld.2011.189-192



APA (American Psychological Association) Style

Rajeev, R., Panda, S. K., Acharya, A. P., Singh, A. P. & Gupta, M. K. (2011) Molecular diagnosis of Haemorrhagic Septicaemia - A Review. Veterinary World, 4 (4), 189-192. doi:10.5455/vetworld.2011.189-192



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