ScopeMed - RSShttp://www.scopemed.orgScopeMed RSSen-us Copyright © 2011 ScopeMed All rights reserved. ScopeMedhttp://www.scopemed.org/img/logo-scopemed-ttk.gifhttp://www.scopemed.org 2016-05-27T20-57-13Z Isolation of endothelial progenitor cells from human adipose tissue http://www.scopemed.org/?mno=230438 2016-05-27T20-57-14Z
Source: Biomedical Research and Therapy
Phuc Van Pham, Ngoc Bich Vu, Hoa Trong Nguyen, Ngoc Kim Phan.
Adipose tissue is a rich source of stem cells, especially mesenchymal stem cells (MSCs). This study aimed to identify and isolate endothelial progenitor cells (EPCs) from human adipose tissue. Belly adipose tissues were collected from donors with consent. Stromal vascular fractions (SVFs) were extracted from adipose tissues by enzyme collagenase using commercial kits. SVFs were cultured in MSCCult medium for 24 h to obtain MSCs, then supernatant was collected and cell pellet cultured in EGM-2 medium to obtain adipose tissue EPCs (ADEPCs). ADEPCs were checked for surface marker expression of CD31 and VEGFR2, and for angiogenesis capability in vitro. The results showed that SVFs contained a pool of EPCs with strong angiogenesis potential and that adipose tissue is not only a source for MSCs but also for EPCs. Therefore, ADEPCs may a useful source of EPCs for vascular medicine.
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Fri, 27 May 2016 19:06:48 GMT -08:00 http://www.scopemed.org/?mno=230438
Effects of ricin extracted from seeds of the castor bean (ricinus communis) on cytotoxicity and tumorigenesis of melanoma cells http://www.scopemed.org/?mno=230436 2016-05-27T20-57-14Z
Source: Biomedical Research and Therapy
Trung Ngoc Ngo, Tho Thi Nguyen, Dung Thi-Thuy Bui, Nhung Thi-My Hoang, Thang Dinh Nguyen.
Ricin has been reported as a potential therapeutic agent for the treatment of various cancers due to its potency. In this study, we succeeded in isolating and purifying total ricin from seeds of the castor bean (ricinus communis) from Vietnam. We also revealed that total ricin showed strong cytotoxicity against melanoma cells; IC50 at 48 h was 34.1 ng/mL for SKMEL28 cells and 5.2 ng/mL for HaCaT cells. We examined the ability of total ricin to inhibit tumorigenesis of SKMEL28 cells in vitro. At low concentrations (< 3ng/mL) total ricin did not cause death of HaCaT or SKMEL28 cells but strongly reduced the size of SKMEL28 tumor colonies formed in soft agar. The effect of ricin on tumorigenesis was also confirmed by apoptotic and immunoblot analyses. Our results showed that treatment with total ricin (3 ng/mL) resulted in decreased ERK and p-ERK expression in SKME28 cells but did not affect expression levels of those genes in HaCaT cells. We showed that, although ricin at 1 and 3 ng/mL did not induce apoptosis of HaCaT cells, it significantly increased apoptosis of SKMEL28 up to 1.4 folds and 2.1 folds, respectively. The results from our study suggest that although ricin is listed as one of the most poisonous substances in nature, it has potential to be used as a drug for melanoma treatment.
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Fri, 27 May 2016 19:05:22 GMT -08:00 http://www.scopemed.org/?mno=230436
Concise review: 3D cell culture systems for anticancer drug screening http://www.scopemed.org/?mno=230435 2016-05-27T20-57-14Z
Source: Biomedical Research and Therapy
Huyen Thi-Lam Nguyen, Sinh Truong Nguyen, Phuc Van Pham.
Three-dimensional (3D) cultures are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than monolayer cultures. In cancer research, the natural tumor characteristics and architecture are more closely mimicked by 3D cell models. Thus, 3D cell cultures are more promising and suitable models, particularly for in vitro drug screening to predict in vivo efficacy. Different methods have been developed to create 3D cell culture systems for research application. This review will introduce and discuss 3D cell culture methods most popularly used in drug screening. The potential applications of these systems in anticancer drug screening will also be discussed.
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Fri, 27 May 2016 19:02:58 GMT -08:00 http://www.scopemed.org/?mno=230435
Is the neutrophil extracellular trap-driven microvascular inflammation essential for diabetes vasculopathy? http://www.scopemed.org/?mno=230434 2016-05-27T20-57-14Z
Source: Biomedical Research and Therapy
Alexander Berezin.
The neutrophil extracellular traps (NETs) are defined as an extensive web consisting decondensed chromatin, which is released from activated neutrophils, as well as cytotoxic proteins, histones and microbicidal proteases that cause tissue damage. NETs contribute to endothelial damage, inflammation, thrombosis, platelet aggregation, ischemia, that are essential players in the pathobiology of diabetic complications. The objective of the review is to highlight the possible role of NETosis in early diabetes-related vasculopathy beyond cardiovascular complications. Although the clinical significance of NETosis in diabetes beyond early atherosclerosis and cardiovascular complications is not still clear, there is limited data with respect to useful to use biological markers of NETosis aimed early stratification of the diabetics at risk of disease progression. Furthermore, several inductors of NETosis might be a target for novel pharmacological approaches to delay advance in diabetes and prevent diabetes-related vasculopathy.
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Fri, 27 May 2016 19:01:01 GMT -08:00 http://www.scopemed.org/?mno=230434
Effect of Desert Camel's Milk and/or Urine on Experimentally Induced Thioacetamide Liver Cirrhosis in Rats http://www.scopemed.org/?mno=230428 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Mohamed M. Zeweil, Ali H. El-Far, Kadry M. Sadek, Usama E. Mahrous, Ahmed S. Ahmed.
The effects of desert camel milk (CM) and desert camel urine (CU) on thioacetamide-induced liver cirrhosis were studied in rats. Therefore one hundred and thirty male rats were allocated into ten groups. The non-cirrhotic control animals were allocated into five groups each consisted of ten rats. The cirrhotic animals were allocated into five groups. Cirrhotic groups were received CM and CU alone or mixed for 12 weeks with an intraperitoneal (IP) thioacetamide (200 mg/kg) injection. Liver damage was assessed by estimation of tissue oxidant malondialdhyde (MDA) and total antioxidant capacity (TAC). Moreover serum levels of total proteins, albumin, globulin and A/G ratio in addition to alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transferase (GGT) and alkaline phosphatase (ALP) activities besides serum bilirubin, cholesterol, triacylglycerols, creatinine and urea were determined. Treatment with CM alone or mixed with CU significantly reduced thioacetamide-induced elevation in tissue MDA as well as serum enzymes (ALT, AST, GGT and ALP), total cholesterol (TC), triacylglycerols (TAG), creatinine and urea. This study gives an evidence that the thioacetamide-induced liver cirrhosis in rats can be ameliorated by administration of CM and CU especially in a dose of 2CM: 1CU.
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Fri, 27 May 2016 13:43:25 GMT -08:00 http://www.scopemed.org/?mno=230428
Mycoplasma Synoviae and other Associated Bacteria Causing Arthritis in Chicken http://www.scopemed.org/?mno=230426 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Rasha G. Tawfik, Samy A. Khalil, Hany F. Ellakany, and Helmy A. Torky.
Arthritis in broiler chickens is one of economic problems causing losses in poultry industry. Hundred samples (60 samples for Mycoplasma synoviae isolation and 40 samples for isolation other bacteria) from chicken with arthritis symptoms, were collected from different broiler chickens farms. The samples were cultivated on PPIO media for isolation of Mycoplasma synoviae and different media( MaCconky , EMB, X.L.D and salt Mannitol media) for isolation of other bacteria and biochemically identified. The results revealed that out of 40 samples 28(70%) were positive for E. coli isolation,10 (25%) were positive for Staphylococcus aureus isolation,2(5%) were positive for Salmonella Enterica isolation. Out of 6osamples 6(10%) were positive for Mycoplasma synoviae isolation. Amplification of vlhA gene of Mycoplasma synoviae showed that out of 6 isolate 3 (50%) were positive for the gene . Six seogroups of E. coli isolates examined serologically and put into six group, one each group (O128, O125, O146,O27,O114 and O158).The result of multiplex PCR for E.coli virulence genes( ibeA and iss ) showed that iss were detected in all serogroups .While ibeA virulence gene was detected in serotypes O125 and O146 only .The result of PCR for amplification of virulance genes of S.aureus(CNA,clfA)reveled that out of 8 isolate of S.aureus,3(37.5%) were positive for clfA. Out of 3 positive S.aureus for clfA,3(100%) were positive for CNA.
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Fri, 27 May 2016 13:27:16 GMT -08:00 http://www.scopemed.org/?mno=230426
Screening for Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes http://www.scopemed.org/?mno=230425 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Khairy M. El-Bayomi, Iman E. El Araby, Ayman, A. Saleh, Hoda Z. Osman, Mahmoud S. Eltarabany, Ashraf Awad, Amir H. Abd El-fattah, and Mohammed Abu El-Magd..
BMP 15 is a member of transforming growth factor-β (TGF- β) superfamily which plays a crucial role in ovarian follicular development and ovulation rate. In this study a partial sequence of this gene was screened for polymorphisms. PCR amplification of DNA isolated from blood of 100 Egyptian buffaloes heifers (50 infertile and 50 normal). Revealed two loci, BMP15.1 (334 bp) which includes exon 1 and partial intron 1 and BMP15.2 (272 bp) which spans partial 5UTR and exon 1. Polymorphisms in these two loci were detected by using direct sequencing and single strand conformational polymorphism (SSCP) technique. A single pattern of SSCP was detected. This was confirmed by nucleotide sequencing which revealed absence of single nucleotides polymorphisms (SNPs) or any other type of polymorphisms in the two loci. The sequencing and PCR-SSCP patterns revealed no polymorphism in all studied animals.
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Fri, 27 May 2016 13:14:22 GMT -08:00 http://www.scopemed.org/?mno=230425
Comparative Efficiency of Different CIDR Protocols for Treatment of Postpartum Anestrous in Egyptian Buffaloes http://www.scopemed.org/?mno=230424 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Rezk S. Ghallab, Fikry M. Hussein, Wael MB. Noseir.
This study aimed to evaluate the efficiency of treatment of postpartum anestrous Egyptian buffaloes in summer season. Protocols of controlled internal drugs (CIDR) as a source of exogenous progesterone were included with some hormonal preparations such as GnRH, PGF2α and estradiol benzoate (EB) for monitoring the resumption of ovarian cyclicity based on assessment of different reproductive performance parameters (EIR, TCR, PR). The study was conducted on 120 postpartum anestrous buffalo-cows, which were diagnosed on basis of case history (>120 days postpartum). Postpartum rectal palpation of both ovaries was performed together with collection of two blood samples with 10 days interval for assessment of serum progesterone level. Buffaloes were assigned to five groups and a control group. Buffaloes in treatment 1 (n=20) received on day (0) GnRH (10μg) +CIDR (inserted intravaginal), 25mg PGF2α on day (7), CIDR was removed on day (8), 2nd dose of GnRH on day (9). Buffaloes in treatment 2 (n=20) received on day (0) GnRH (10mg)+CIDR, CIDR was then removed+2nd dose of GnRH on day (7). Buffaloes in treatment 3 (n=20) received CIDR on day (0), CIDR was removed+PGF2α (25mg) injected on day (7). Buffaloes in treatment 4 (n=20) CIDR inserted +GnRH (10mg) on day (0), CIDR removal+25mg PGF2α on day (7), 24hrs EB was injected. Buffaloes in treatment 5 (n=20) CIDR for 7 days then removed. Buffaloes in control group (n=20) had no treatment. Results in treatment 1 (P4, 0.86±0.1) were; EIR (80%), TCR (84.2%) and PR (80%). While, in treatment 2 (P4, 0.86±0.10) EIR (55%), TCR (68.42%) and PR (65%). Results in treatment 3 at P4 level (0.79 ±0.10) were; EIR (70%), TCR (72. 2%) and PR (65%). Also, in treatment 4 (P4, 0.87±0.10) EIR (85%), TCR (78.9 %) and PR (75%). While in treatment 5 (P4, 0.85±0.07) were; EIR (45%), TCR (57.14 %) and PR (40%). In control (group 6) at P4 level (0.93 ±0. 09) the results were; EIR (15%), TCR (33.3%) and PR (5%). In conclusion, the results indicated that addition of GnRH and PF2α to progesterone based CIDR protocol (CIDR-ovsynch) substantially improves the EIR, TCR and PR in postpartum anestrous buffaloes than CIDR protocol alone and other treated groups in comparison to control.
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Fri, 27 May 2016 13:08:36 GMT -08:00 http://www.scopemed.org/?mno=230424
Comparison between Bacteriological Isolation and Molecular Detection of E. coli from Chickens Suffering from Colibacillosis and/or Diarrhea http://www.scopemed.org/?mno=230413 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Reem Alnahass, Samy A. Khaliel, Hany F. Ellakany, Madiha S. Ibrahim.
In the current study, we compared the efficiency of PCR detection to culture-dependent isolation of E. coli, as a model, from the liver and intestinal contents. Further, the incidence differences of E. coli isolation from the liver and intestine. Samples were collected from birds suffering from respiratory manifestation and/or diarrhea. Ninety E. coli isolates were recovered from 60 birds (52 intestinal and 38 liver samples) by bacteriological culture on selective broth and selective agar. PCR was performed using pho-A gene as a general marker for E. coli on DNA directly purified from the samples and assigned PCR (a). Negative PCR (a) samples were cultured on broth and another PCR was done, assigned PCR (b). Bacteriological isolation was more sensitive than PCR (a) indicating that inhibitors in the samples could have reduced or totally blocked the amplification capacity of PCR (a), which limited its diagnostic usefulness. PCR (b) was more sensitive than PCR (a) and more practical than bacteriological isolation. Detection of three virulence genes; iut-A, iss and tsh, showed genotypic variations of avian pathogenic E. coli (APEC) isolated from the liver and the intestine. In conclusion, an enrichment step results in more sensitive PCR than culture performed one. Further, general genes rather than virulence genes should be used as an indicator for E. coli detection in infected materials.
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Fri, 27 May 2016 10:03:17 GMT -08:00 http://www.scopemed.org/?mno=230413
Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals http://www.scopemed.org/?mno=230412 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Ismail. I. Abo-Ghanema , Madiha S. Ibrahim , Bahig. R. Nemettallah , Hanan A. Ghoneim.
A total of 10 Staphylococcus aureus isolates were recovered from 117 milk samples which were obtained from 93 dairy cows indifferent farms suffered from clinical mastitis, these isolates were phenotypically characterized and identified based on colony morphology andbiochemical tests. In addition, antibiotic susceptibility pattern of these strains was investigated and a trial to investigate the mechanism of the isolated bacterium to antibiotic resistance by PCR detection of class 1 integron. The milk samples were examined bacteriologically: Thirty-three out of 117 samples (28.2%) were positive for staphylococcus. Ten out of 33 samples (30.3%) were coagulase positive staphylococcus. The S. aureus isolates were highly sensitive to Cephalothin (DF30), Ampicillin/sulbactam (SAM), Methicillin(DP5µg), Amoxicillin-Clavulanic acid (AMC30) and Trimethoprime-Sulphamethaxole (SXT25µg), Susceptibility rates of S. aureus to these antibiotics were: 90, 80, 80, 70 and70%, respectively. Higher resistance was observed to Streptomycin (S10µg), Kanamycine (K30µg), Tetracycline (Te30µg), Erythromycin(E15µg), Cefotasime (CTX30µg), Cefoperazone(CFP75µg), Ampicillin (AMP10µg), Gentamycine (GN10µg), Amoxicillin (AX25µg) and Ciprofloxacin(CIP5µg), Susceptibility rates of S. aureus to these antibiotics were: 30, 30, 30, 30, 40, 40, 40, 40,40 and 50%, respectively. The molecular detection of S. aureus specific sequence gene(Sa), genes encoding clumping factor A(clfA), gene encoding S. aureus protein A (spa)and the class 1 integron using Polymerase Chain Reaction were carried out. A total of 10 Staphylococcus aureus isolates were negative for the presence of class 1 integron but yielded an amplicon size of about107 bp in case of S. aureus specific sequence gene (Sa), an amplicon size of about 638bp in case ofclfA gene. Amplification of the(spa) gene encoding S. aureus protein A was detected in 9 strains out of 10 (90%)and yielded an amplicon size of about 226bp. The data in this study provided an overview on the incidence of staphylococcus aureus in clinical mastitis and the distribution of virulence determinants and antibiotic resistance of these S. aureus strains.
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Fri, 27 May 2016 09:55:21 GMT -08:00 http://www.scopemed.org/?mno=230412
Identification of Different DermatophytesIsolated From Cattle, Cats and Horses Suffered From Skin Lesions http://www.scopemed.org/?mno=230410 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Wagdy R. ElAshmawy* and Mohamed E. Ali.
Dermatophytes are considered one of the important zoonotic superficial fungal skin diseases in different animal species in the world especially among immunocompromised, stressed and senile individuals. The disease affects the animal productivity, leather industry (cattle), animal performance (Horses) and zoonotic importance (cats).our study focused on three main species cattle, horses and cats with skin lesions suspected to be ring worm to determine the different causes. Diagnosis was carried out using direct examination of skin scraping, isolation of the fungus using FungAssay and microscopic determination of fungal spores and microconidia. Different species of dermatophytes were isolated from calves including, Microsporumcanis and Microsporum gypsum, while in horses Trichophytonmentagrophytes and Microsporum gypsum were isolated and in cats Trichophytonmentagrophytes and Microsporumcanis were isolated. Mixed infection was reported in some cases. We could conclude that the isolated fungi are Microsporumcanis,Trichophytonmentagrophytes and Microsporum gypsum.
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Fri, 27 May 2016 09:49:30 GMT -08:00 http://www.scopemed.org/?mno=230410
Diagnosis and Treatment of Bacterial Endometritis in Arabian Mares http://www.scopemed.org/?mno=230409 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Haitham A. Barbary, Ismail I. Abo-ghonema, Iman E. El-Bawab, Moustafa S. Fadel.
Endometritis remains a major clinical problem in broodmare practice. The diagnosis of endometritis is based on the presence of inflammation, the mare’s history, physical and reproductive examination including transrectal ultrasonography and laboratory diagnostic aids. In this study, eighteen repeat breeder mares were suspected to be suffering from endometritis through their examination by ultrasonography. A total of 18 uterine swabs from these mares were evaluated and the bacterial isolates were identified. The bacterial species most frequently isolated was E. coli (9 isolates, 50%), Staphylococcus aureus (5 isolates, 27.8%) and Klebsiella pneumoniae (3 isolates, 16.7%),citrobacter freundii and Streptococci (2 isolates, 11.1%). The study also revealed that other Klebsiella oxytoca, Providencia alcalifaciens and Proteus mirabilis were found in the uterine culture but in very lower frequencies. Ciprofloxacin and enrofloxacin was the most effective antimicrobial agent inhibiting most of uterine bacterial isolates in our work.
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Fri, 27 May 2016 09:41:48 GMT -08:00 http://www.scopemed.org/?mno=230409
Protective Role of Alpha Lipoic Acid against the Deleterious Effects of both Natural and Artificial Sweetener (Sucrose and Aspartame) in Albino Rats http://www.scopemed.org/?mno=230407 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Hamdy B. Gafar, Nabil Taha, Saad N. Noeman, Abdelwahab Mandour, Mohamed Lepda.
This study was planned to throw light on the adverse effect of natural sweetener (sucrose) and artificial sweetener (aspartame) and the possible way of alpha lipoic acid to ameliorate the deleterious effects which may produce from these sweeteners in rats. There is a significant increase of alkaline phosphatase activity of sucrose treated group in comparison to untreated one at 45and 90- day of the experiment at (P < 0.01). Administration of lipoic acid did not reduce the potential effect of sucrose. On the other hand, aspartame had no effect on the level of alkaline phosphatase either at 45-day or at 90-day of the experiment. Sucrose had no effect on the level of total serum protein at 45-day and 90-day of the experiment. Administration of lipoic acid in combination of aspartame was significantly increase the level of serum total protein at 45 day of the experiment and had no effect at 90 day of the experiment. Sucrose was significantly decreased the level of albumin /globulin at 45 days of the experiment Administration of sucrose or aspartame was significantly increased level of urea only at 90-dayof the experiment in comparison to untreated group. Lipoic acid in combination of sucrose or aspartame was significantly decreased level of urea at 90-day of the experiment in comparison to sucrose or aspartame treated group. Administration of sucrose or aspartame was significantly increased level of serum creatinine at 90-day of the experiment in comparison to untreated group. Administration of aspartame was significantly decreased and increased the level of serum uric acid at 45 and 90 days respectively while sucrose decreased uric only at 45 day of the experiment in comparison to untreated group. Administration of sucrose or aspartame was significantly increased level of cholesterol and triglycerides during the experiment in comparison to untreated group. Administration of lipoic acid in combination of sucrose or aspartame was significantly decreased level of cholesterol during the experiment in comparison to sucrose or aspartame treated group. Sucrose was significantly increased level of glucose during the experiment in comparison to untreated group. Lipoic acid in combination of sucrose was significantly decreased level of glucose during the experiment in comparison to sucrose treated group. Lipoic acid in combination of aspartame was significantly decreased level of glucose at 45-day of the experiment but had no effect at 90-day of the experiment in comparison to aspartame treated group.
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Fri, 27 May 2016 09:36:03 GMT -08:00 http://www.scopemed.org/?mno=230407
Treatment Trails of Saprolegniosis in Oreochromis Niloticus http://www.scopemed.org/?mno=230406 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Ahmed H. Sherif, and Shehata, A. Abdel–Hakim.
This study was performed to compare between different methods of Saprolegnia treatment hydrogen peroxide H2O2, potassium permanganate KMnO4 and biological treatment non pathogenic bacterial strain Aeromonas hydrophila (NPAH). 120 Oreochromis niloticus were randomly collected and investigated for Saprolegnia fungal infection and infection rate were 10%, 5% and 15% in tolompate village, Elhamool and om-sin village respectively. Obtained Saprolegnia parasitica (S. parasitica) was examined by Polymerase chain reaction (PCR of ITS gene, 750 bp). Results obtained revealed that Kmno4 had the highest effective treatment in S. parasitica infection compared with biological and control treatment. Also, biological treatment with A. hydrophila showed hazourd of bacterial infection. So, KMnO4 recommended in case of S. parasitica infection as treatment of choice followed by H2O2 and a biological treatment with A. hydrophila is not recommended.
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Fri, 27 May 2016 09:26:30 GMT -08:00 http://www.scopemed.org/?mno=230406
Genotypic Molecular Detection of Certain Genes Encoding Virulence Determinates and Antibiotic Resistance in Staphylococcus Aureus Isolates from Mastitis Cows http://www.scopemed.org/?mno=230404 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Ahmed L. Al-Ashmony, Abd-AlSlam F. Al-Sawy, Helmy A. Torky.
A total of 10Staphylococcusaureusisolates were recovered from 117 milk samples which were obtained from 93 dairy cows indifferent farms suffered from clinical mastitis, these isolates were phenotypically characterized and identified based on colony morphology andbiochemical tests. In addition, antibiotic susceptibility pattern of these strains was investigated and a trial to investigate the mechanism of the isolated bacterium to antibiotic resistance by PCR detection of class 1 integron. The milk samples were examined bacteriologically: Thirty-three out of 117 samples (28.2%) were positive for staphylococcus. Ten out of 33 samples (30.3%) were coagulase positive staphylococcus. The S. aureus isolates were highly sensitive to Cephalothin (DF30), Ampicillin/sulbactam (SAM), Methicillin(DP5µg), Amoxicillin-Clavulanic acid (AMC30) and Trimethoprime-Sulphamethaxole (SXT25µg), Susceptibility rates of S. aureus to these antibiotics were: 90, 80, 80, 70 and70%, respectively. Higher resistance was observed to Streptomycin (S10µg), Kanamycine (K30µg), Tetracycline (Te30µg), Erythromycin (E15µg), Cefotasime (CTX30µg), Cefoperazone (CFP75µg), Ampicillin (AMP10µg), Gentamycine (GN10µg), Amoxicillin (AX25µg) and Ciprofloxacin(CIP5µg), Susceptibility rates of S. aureus to these antibiotics were: 30, 30, 30, 30, 40, 40, 40, 40,40 and 50%, respectively. The molecular detection of S. aureus specific sequence gene(Sa), genes encoding clumping factor A (clfA), gene encoding S. aureus protein A (spa)and the class 1 integron using Polymerase Chain Reaction were carried out. A total of 10 Staphylococcus aureus isolates were negative for the presence of class 1 integron but yielded an amplicon size of about107 bp in case of S. aureus specific sequence gene (Sa), an amplicon size of about 638bp in case ofclfA gene. Amplification of the(spa) gene encoding S. aureus protein A was detected in 9 strains out of 10 (90%)and yielded an amplicon size of about 226bp. The data in this study provided an overview on the incidence of staphylococcus aureus in clinical mastitis and the distribution of virulence determinants and antibiotic resistance of these S. aureus strains.
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Fri, 27 May 2016 09:16:53 GMT -08:00 http://www.scopemed.org/?mno=230404
Evaluation of phenotypic tests for detection of Amp C beta-lactamases in clinical isolates from a tertiary care hospital of Rawalpindi, Pakistan http://www.scopemed.org/?mno=230401 2016-05-27T20-57-14Z
Source: Journal of the Pakistan Medical Association
Nadia Saad, Tehmina Munir, Maliha Ansari, Mehreen Gilani, Mahwish Latif, Amira Haroon.
Objective: To evaluate sensitivity and specificity of disc approximation test compared to three-dimensional extract test as a phenotypic gold standard test for detection of AmpC beta-lactamase producing Escherichia coli and Klebsiella pneumoniae. Method: The cross-sectional validation study was conducted from November 2014 to April 2015 at Army Medical College, Rawalpindi, Pakistan. Extended spectrum beta lactamases (ESBLs) were isolated from various clinical specimens. Screening for AmpC beta-lactamases was done by using cefoxitin disc (30µg) showing inhibition zone diameter of
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Fri, 27 May 2016 08:57:11 GMT -08:00 http://www.scopemed.org/?mno=230401
Study of the pattern of mortality caused by Traffic Accidents (TAs) in The South of Iran http://www.scopemed.org/?mno=230400 2016-05-27T20-57-14Z
Source: Journal of the Pakistan Medical Association
Tayebeh Rakhshani, Fatemeh Rakhshani, Zahra Sadat Asadi, Mohammad Hadiabasi, Kazem Khorramdel, Mohammad Zarenezhad.
Objective: To investigate the pattern of mortality caused by road traffic accidents and its associated factors. Methods: The retrospective cross-sectional study was conducted in the Fars province of Iran and comprised data of all people having died in road traffic accidents from March 21, 2011, to March 19, 2012. Using census method, all files related to deaths in road accidents were reviewed at the Legal Medicine Centre. Data was analysed using SPSS20. Results: Of the 1668 people dead, 1291(77.4%) were male and 377(22.6 %) were female. The highest rate related to the age group 21-30 with 454(27%) killed. As for education level, the highest rate of mortality was 410(24.5%) for illiterate people and the least was 9(0.5%) for those having Masters degree or higher. In terms of time of year, the highest rate of mortality was 167(10%) between August 23and September 22, while the least was 82(5%) between July 23and August22. Head trauma was reported as the most important cause of death in 1191(71.4%) cases. Conclusion: Despite some minor differences, all provinces in Iran have the same patterns of death related to road traffic accidents.
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Fri, 27 May 2016 08:35:52 GMT -08:00 http://www.scopemed.org/?mno=230400
Implementing evidenced based patient safety practices http://www.scopemed.org/?mno=230399 2016-05-27T20-57-14Z
Source: Journal of the Pakistan Medical Association
Ali Yawar Alam.

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Fri, 27 May 2016 08:20:37 GMT -08:00 http://www.scopemed.org/?mno=230399
New perspectives in the management of diabetic retinopathy http://www.scopemed.org/?mno=230398 2016-05-27T20-57-14Z
Source: Journal of the Pakistan Medical Association
M. A. Rehman Siddiqui, M. Amer Raza Awan.

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Fri, 27 May 2016 08:19:06 GMT -08:00 http://www.scopemed.org/?mno=230398
Some Behavioural and Hormonal Study on Egyptian Balady Goat http://www.scopemed.org/?mno=221959 2016-05-27T20-57-14Z
Source: Journal of Advanced Veterinary and Animal Research
Azhar Fakhry Abdel Fattah, Shereen El sayed Abdel-Hamid.
The objective of present study was determine whether the effect of parity of female goat, social ranking and ovulatory responses of does during seasonal anestrous period by exposure to males effect. The study was conducted between November 2014 and January 2015. A study was carried out on a flock of Baladi goats belonging to the farm of Faculty of Veterinary Medicine, Zagazig University. 50 baladi goats, apparently healthy, aged 12 – 18 months were used. (4 males, 50 females). Animals in indoor system were released to graze during daylight hours and returned to closed shed. The goats were fed in a manger with a small cylindrical concentrate pellets applied in small amount. The obtained results revealed that the mounting were higher in males in contact with multiparouse females than those of nulliparouse ones. As the sexually experienced does induce the activation of L.H hormones leading to stimulation of oestrous responses of does. The social ranking of does had a significant effect on sexual behaviour of bucks when exposed to does during anoestrus period. L.H hormones secretion in 2nd week in female exposure to males was higher than the 1st week of exposure at both multiparous and nulliparous females. It could be concluded that visual, alfactactory, auditory contact with the male stimulate oestrous response of anestrous females thus induce the oestrous synchonization resulting in an increase in the economic gain of goat farm.
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Fri, 27 May 2016 06:32:48 GMT -08:00 http://www.scopemed.org/?mno=221959
Maxillary sinüs mucocele presenting with proptosis: A case report http://www.scopemed.org/?mno=227866 2016-05-27T20-57-14Z
Source: Medicine Science | International Medical Journal
Mehmet Kelles, Yuksel Toplu, Mehmet Bozoglu, Soner Demirel, Erkan Karatas, Ahmet Kizilay.
Mucocele is a benign, cystic lesion of the paranasal sinus which grows slowly and becomes symptomatic after a long time. As its size increases the lesion expands out of the sinus. We reported a 16-year-old patient who presented with complaints such as left periorbital pain, shifting in the left eye and proptosis. Mucocele is most commonly seen in the frontal and ethmoid sinuses, while it is rare in the maxillary and sphenoid sinuses. We presented a case of maxillary sinüs mucocele with orbital complaints.
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Fri, 27 May 2016 01:22:03 GMT -08:00 http://www.scopemed.org/?mno=227866
Urinary retention in male patient associated with aripiprazole http://www.scopemed.org/?mno=225375 2016-05-27T20-57-14Z
Source: Medicine Science | International Medical Journal
Lara Utku Ince, Lale Gonenir Erbay, Suheyla Unal.
Urinary retention and / or voiding difficulty are a rare side effect caused by the second generation antipsychotics. Aripiprazole has a lower incidence of adverse effects than other second generation antipsychotics (SGA) and aripiprazole can be used as an alternative treatment on patients who have urinary retention depending on antipsychotic use. Although there are limited data in literature about the effect of aripiprazole on urinary retention, a case was encountered urinary retention with the use of antidepressants and aripiprazole together. Also there is only one case in the literature about urinary retention occurred with aripiprazole. Our case emerged urinary retention with the addition of aripiprazole while under treatment of paroxetine. Patient’s complaints have stopped with the discontinuation of aripiprazole and never emerged under treatment of paroxetine and risperidone. We aimed to contribute this case because there is limited data in literature about this subject. As a result, adrenergic, cholinergic, serotonergic, dopaminergic and histaminergic pathways have role on control of micturition but it is not clear that on which pathway aripiprazole acts on urination. However there is no symptom of urinary retention after stopping aripiprazole and adding risperidone, shows that there is a different mechanism associated with voiding in this case. Additional studies need to be done to clarify the mechanism.
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Fri, 27 May 2016 01:01:54 GMT -08:00 http://www.scopemed.org/?mno=225375
A Six (6) Month Survey of Gross Pathological Conditions of Slaughtered Cattle at Yola Abattoir Adamawa State, Nigeria http://www.scopemed.org/?mno=225369 2016-05-27T20-57-14Z
Source: International Journal of Livestock Research
Mohammed Baba Ardo, Shadrach Kevin Ndyakalah and Haruna Lawal.
A cross sectional study was conducted from November, 2014 to April, 2015 on 14,508 slaughtered cattle at Yola abattoir Adamawa State on Postmortem examination. The objectives of the study were to determine the prevalence of Contagious Bovine PleuroPneumonia (CBPP), Bovine Fasciolosis and Bovine Tuberculosis (BTB) in slaughtered cattle according to sex and breeds. A regular visit was made to the Yola Abattoir at 6:00 AM Seven (7) times a week and this was done for Six (6) Months. A total of 14,508 slaughtered cattle were examined at postmortem and 3,418 (23.56%) cases were recorded; CBPP 898 (6.19%), Fasciolosis 1,100 (7.58%) and TB 1,420 (9.79%). There was Statistical significance (P
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Fri, 27 May 2016 00:50:00 GMT -08:00 http://www.scopemed.org/?mno=225369
Non-invasive evaluation of liver stiffness in nonalcoholic fatty liver disease: supersonic shear imaging, FibroScan or ARFI? http://www.scopemed.org/?mno=226076 2016-05-27T20-57-14Z
Source: Medicine Science | International Medical Journal
Coskun Ozturker, Ergenekon Karagoz, Ersin Ozturk.

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Fri, 27 May 2016 00:46:12 GMT -08:00 http://www.scopemed.org/?mno=226076
“DAILY SPERM PRODUCTION RATE AND SERUM TESTOSTERONE HORMONE LEVEL IN BUCKS (Capra hircus) DURING BREEDING AND NON-BREEDING SEASONS” http://www.scopemed.org/?mno=220338 2016-05-27T20-57-14Z
Source: International Journal for Agro Veterinary and Medical Sciences
Harshilkumar Ratilal Thakor.
Twelve bucks were taken for experiment. The animals were divided into two groups (breeding season and non-breeding season) of six animals each. In breeding season the daily sperm production rate was 1.35 ± 0.07 billion with an average of 1.28 ± 0.06 billion for the left testes and 1.41 ± 0.08 billion for the right testes while in non-breeding season the daily sperm production rate was 1.05 ± 0.06 billion with an average of 0.95 ± 0.08 billion for the left testes and 1.15 ± 0.10 billion for the right testes. Highly significant (P < 0.01) difference was observed between seasons for DSPR. In breeding season plasma testosterone concentration was 1.24 ± 0.07 ng/ml while in non-breeding season plasma testosterone concentration was 1.03 ± 0.06 ng/ml. Highly significant (P < 0.01) difference was observed between seasons for plasma testosterone levels.
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Thu, 26 May 2016 22:45:43 GMT -08:00 http://www.scopemed.org/?mno=220338
IMPLICATION OF PROLONGED ADMINISTRATION OF TRAMADOL ON REPRODUCTIVE PERFORMANCE OF MALE WISTAR RATS http://www.scopemed.org/?mno=215004 2016-05-27T20-57-14Z
Source: International Journal for Agro Veterinary and Medical Sciences
OLADEJO AYODELE OLAOLU, AJALA OLUWATOYIN, OLOYE ABIMBOLA ADETOKUNBO, AJANI OLUMIDE SAMUEL.
Tramadol is a centrally active synthetic opioid analgesic that is used extensively in abolishing pain in animals. The effect of prolonged administration of tramadol on some reproductive parameters of wistar strain albino rats was studied. Fifteen sexually matured male albino rats weighing between 150 – 200 g were divided into three groups (A, B and C) of five rats in each. Group A (control) was given distilled water while the experimental groups B and C were treated orally with 50mg/kg and 100mg/kg of tramadol respectively for 28 days. The hormone profile of groups B and C male rats showed a significant decrease (p
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Thu, 26 May 2016 22:45:20 GMT -08:00 http://www.scopemed.org/?mno=215004
Retention of Fetal Membranes and its effect on Puerperium in Bovines: A Review http://www.scopemed.org/?mno=214774 2016-05-27T20-57-14Z
Source: International Journal for Agro Veterinary and Medical Sciences
R. V. Patel, Sanjay C. Parmar.
Retention of the fetal membranes (RFM) or Retention of Placenta (ROP) in the cow is normally defined as the condition in which the fetal membranes are not expelled within a period of 12 hours after expulsion of the fetus. Primary retention of fetal membranes results from a lack of detachment from the maternal caruncles whereas secondary retention is related to a mechanical difficulty in expelling already detached fetal membranes. There are a number of risk factors associated with RFM, including induced parturition, shortened gestation, abortion, twinning, dystocia, fetotomy, cesarean section, nutritional deficiencies such as vitamin E, selenium and carotene, infectious agents such as bovine viral diarrhea virus and immunosuppression. The most commonly used hormonal products in treating RFM are prostaglandins and oxytocin and these hormones play a role in uterine contraction and could be effective in treating retention of the fetal membranes.
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Thu, 26 May 2016 22:44:59 GMT -08:00 http://www.scopemed.org/?mno=214774
Review on Epidemiology of Clinical and Sub clinical Mastitis on Dairy cows http://www.scopemed.org/?mno=212102 2016-05-27T20-57-14Z
Source: International Journal for Agro Veterinary and Medical Sciences
Amare Argaw Arata, Reta Duguma.
Review on Epidemiology of Clinical and Sub clinical Mastitis on Dairy cows Amare Argaw¹ Reta Duguma² ¹ Southern Agricultural Research Institute ²Addis Ababa University College of Veterinary Medicine and Agriculture SUMMARY Mastitis is a complex and multi-factorial disease, the occurrence of which depends on variables related to the animal, environment and pathogen. It reduces the quality and quantity of milk, and is one of the most important and expensive disease of dairy industry. Among the pathogens, bacterial agent are the most common one, the greatest share of which resides widely distributed in the environment of dairy cows, hence a common threat to the mammary gland. Mastitis is very common in cows of both developed and developing countries. Based on clinical symptoms, it can be classified into two types, namely, clinical and subclinical mastitis. Clinical mastitis is often diagnosed directly by visual assessment of udder inflammation or by changes in milk’s organoleptic properties whereas, Subclinical mastitis is non observable form of mastitis, which is with no visible abnormalities of either the milk or the udder and is characterized by sudden rise in milk somatic cell count. Mastitis pathogens can be divided into contagious and environmental. The primary reservoir of con¬tagious pathogens is an infected udder whereas a contaminated environment is the primary reser¬voir of pathogens causing environmental mastitis. As in the rest part of the world, mastitis is one of the most economically important disease of dairy sector and is an important factor that limits dairy production in Ethiopia, giving emphasis only to clinical mastitis and subclinical mastitis, (with prevalence of 23% to 85%) is with little attention, causing heavy financial loses and public health hazards. Early diagnosis of mastitis is vital because changes in the udder tissue take place much earlier before they become apparent. Many factors influence the incidence of mastitis, such as Age, parity and lactation stage of a cow, lactation number, herd management, husbandry environment, temperature, humidity, seasons, breeds, and milking characteristics, and nature of the pathogen. Antibiotic therapy is usually prescribed when clinical symptoms of mastitis are presented. If detected early, antibiotic therapy is very effective in curing and controlling the spread of contagious pathogens. However, The use of antimicrobials have, over time, increased the number of antimicrobial-resistant microbes globally, and any use of these agents will to some extent benefit the development of resistant strains and also inappropriate usage of antimicrobials such as wrong dose, drug or duration may contribute the most to the increase in antimicrobial resistance without improving the outcome of treatment. Key words: Mastitis, Ethiopia, dairy cows, antibiotic
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Thu, 26 May 2016 22:44:39 GMT -08:00 http://www.scopemed.org/?mno=212102
Role of ultrasound in diagnosis of testicular pathology of Yankasa rams experimentally infected with Fasciola gigantica in Zaria, Nigeria. http://www.scopemed.org/?mno=212187 2016-05-27T20-57-14Z
Source: International Journal for Agro Veterinary and Medical Sciences
Dauda Iliyasu, Rwuaan sackey Joseph, Chiezey N Paulin, Ate I Utem, maruf lawal, Auwal Usman, Ahmed idris, Ajani J Ayinla.
The study was designed to evaluate the role of ultrasound in diagnosis of testicular organ of Yankasa rams experimentally infected with fasciola gigantica. Twelve (12) Yankasa rams with average body weight of 21.3±0.3 kg were randomly assigned into two groups, infected group (n=7) and control group (n=5). The infected group were orally inoculated with 800 metacercariae and monitored for 2 weeks for manifestation of clinical signs. The rams were examine using ultrasound at 3, 5, 9 and 11 weeks post infection (PI) to determine the level of damage on the testes. At week 9 and 11 (PI) ultrasonograms of the testes of the infected rams (n=7) showed loss of consistency of the testicular morphology and hyperechoic appearance which were indication of testicular degeneration. The aim of the study was to employ the use of ultrasound machine in diagnosis of testicular pathology following experimental fasciola gigantica infection in Yankasa rams.
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Thu, 26 May 2016 22:44:20 GMT -08:00 http://www.scopemed.org/?mno=212187
Prognostic factors affecting overall survival in right colon cancer http://www.scopemed.org/?mno=211037 2016-05-27T20-57-14Z
Source: Archives of Clinical and Experimental Surgery (ACES)
Mehmet Ince, Nail Ersoz, Mehmet Fatih Can, Gokhan Yagci, Sezai Demirbas, Ismail Hakki Ozerhan, Yusuf Peker.
Background: The prognosis of patients who have carcinoma of the colon is dependent on several factors that are clinical, pathological, and biological. Adequate lymph node staging in patients with colon cancer is important for determining prognosis and planning further treatment. We aimed to determine what factors might predict survival in patients with right colon cancer. Methods: Between 2007 and 2014, consecutive patients undergoing operation for adenocarcinoma of the right-sided colon were enrolled in this study. The following factors were analyzed with the Cox regression model: age, gender, localization of the tumor, recurrence, pTNM stage, removed and invaded lymph node status (MLN) and survival rate. Multivariate models were used to assess the adjusted effects and to explore the interaction between survival and other factors. Results: A total of 56 (38,1%) men and 91 (61,9%) women, mean age being 61,8 ± 15,9 years, were included. The mean survival time was 46,5 ± 43,2 months. The mean LN number was 18,8 ± 9,44; MLN number was 2,66 ± 5,13. Age, total LN, MLN number and postoperative stage were significant in the univariate analysis for survival. Independent predictors of survival in multivariate analysis were age (p=0,019), postoperative stage (p=0,039), and MLN (p=0,003). Conclusions: LN metastasis is a prognostic feature in patients by means of colon cancer. It could not be changed independent of a number of prognostic factors, such as age, but LN number was dependent on operation in right colon cancer surgery. LN yields have been linked to improved survival and its determination is reliant on both the surgeon and the pathologist.
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Thu, 26 May 2016 14:34:12 GMT -08:00 http://www.scopemed.org/?mno=211037
Ostrich Pox Virus Infection in Farms at Some Northern Egyptian Governorates http://www.scopemed.org/?mno=230329 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Moshira, A. El-Abasy, Fares, F. El-khyate, Samy, A. Adayel, Hefny, Y. Hefny and Abd-Elgelil, A. El-Gohary.
This work was aimed to study the epidemiology and possible control ways of ostrich pox virus in Egypt during the period from 2011-2012. For this purpose, virological examination of 33 skin samples from 429 diseased ostriches and their premises (12 samples from feather and 10 samples from mosquitos). Samples were collected from diseased ostriches of all ages suffered from dry and wet pox lesions from 11 ostriches farms at Sharkia, Kalubia and Ismailia Governorates and used for isolation of ostrich pox virus. The diseased ostriches were subjected to clinical, postmortem, serological and histopathological examinations. All pock lesion induction samples were positive for agar gel precipitation test (AGPT) for pox virus. Experimental infection of 6- week-old turkey poults by the isolated ostrich pox virus (1x106.5 PFU/ml) from skin lesions, (1 x 107.3 PFU/ml) from feathers and (1.5 x 107.5 PFU/ml) from mosquitoes revealed high pathogenicity to dry and wet lesions, histopathological changes and sero-conversion. Experimental infection of 9- week-old ostrich previously vaccinated at 6-week-old with live attenuated fowl pox vaccine revealed no clinicopathogical effects while the experimentally infected unvaccinated ostriches revealed high pathogenicity to dry lesions. In conclusion, early vaccination of ostriches with live attenuated fowl pox vaccine at 6-week-old with the control of mosquitos prevented the appearance of natural ostrich pox virus infection.
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Thu, 26 May 2016 13:40:18 GMT -08:00 http://www.scopemed.org/?mno=230329
Serological, Rapid Molecular Characterization and Antibiotic Resistance for Field Isolates of Mycoplasma Gallisepticum in Chicken in Saudi Arabia http://www.scopemed.org/?mno=230328 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Ayman Elbehiry, Musaad Al-Dubaib, Eman Marzouk.
Mycoplasma Gallisepticum (MG) is considered as one of the most economically important mycoplasmal pathogens among poultry industry worldwide. This pathogen has various strains, therefore their detection using culture method is not sufficient. From this point of view, this study was designed to develop a novel TaqMan® real-time polymerase chain reaction (TaqMan RT-PCR) assay for direct detection of MG using cytadhesin to encode a surface protein (mgc2) containing a TaqMan FAM-labeled minor groove binder probe that targets this gene and to compare it with conventional polymerase chain reaction and immunological methods. Using serial broth dilution methods against identified MG isolates, minimum inhibitory concentrations of various antimicrobial agents were detected. Throughout Al-Qassim region, Kingdom of Saudi Arabia, 208 specimens were collected from 18 commercial chicken broiler farms where respiratory diseases were present. Furthermore, 180 blood serum samples were investigated for serological diagnosis of MG. Serum plate agglutination and enzyme-linked immunosorbent assay techniques detected positive serological identification in 83 (46.11%) and 97 (53.88%) isolates, respectively. The sensitivity recorded for TaqMan RT-PCR was 10–3 CFU/ml for MG template DNA. Results of TaqMan RT-PCR revealed 169 positive samples (81.25%), while 108 samples (51.92%) were identified as positive through conventional polymerase chain reaction assay. The results of MIC for 11 antimicrobial agents against 60 identified MG isolates indicated that all groups of MG exhibited a higher degree of sensitivity to tiamulin (93.33%) at low level of MIC50 and MIC90 (≤0.032 µg/ml) followed by tylosin (85%) and doxycycline (81.66%) with MIC50 and MIC90 ranged from ≤0.032 to 2 µg/ml; In contrast, gentamycin, tilmicosin, erythromycin, ciprofloxacin, oxytetracylcine and enrofloxacin did not show a higher activity at low concentrations. In conclusion, the developed TaqMan RT-PCR exhibited higher sensitivity and applicable accuracy than other molecular and serological techniques and thus could be highly recommended for the management of MG susceptibility and to facilitate implementation of effective treatment and prophylactic measures.
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Thu, 26 May 2016 13:31:52 GMT -08:00 http://www.scopemed.org/?mno=230328
Seasonal Variations of Raw Milk Hygienic Quality in Saudi Arabia Dairy Herds http://www.scopemed.org/?mno=230327 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Ahmed M. Elmoslemany, Sohail F. Almuhanna and Abdulmohsen A. Alnaeem.
This study was conducted to evaluate current bacterial quality of raw milk in Saudi Arabia dairy farms, to determine correlation among milk quality tests and to investigate seasonal variations in raw milk bacterial counts. Farm records on bacterial quality of raw milk were obtained from one of the largest dairy processor in Saudi Arabia over one year period (January to December 2013). Bulk tank milk samples were tested for total bacterial (TBC), coliform (CC) and laboratory pasteurization (LPC) counts by dairy plant on daily basis. The results showed that the mean and median values were 5,234 and 1,700 cfu/ml for TBC, 738 and 160 cfu/ml for CC, and 87 and 11 cfu/ml for LPC, respectively. There was low to moderate correlation among bacterial counts, indicating different sources of contamination. Seasonal data indicate higher counts during late fall and early winter compared to other months. In conclusion, this study provide insight on the current level of raw milk bacterial quality in Saudi Arabia and emphasize the importance of using multiple quality parameters for evaluation of raw milk hygienic quality.
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Thu, 26 May 2016 13:24:02 GMT -08:00 http://www.scopemed.org/?mno=230327
Growth Performance, Immune Response, Blood serum parameters, Nutrient Digestibility and Carcass Traits of Broiler Chicken as Affected by Dietary Supplementation of Garlic Extract (Allicin) http://www.scopemed.org/?mno=230326 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Mohamed I. El-katcha, Mosad.A. Soltan, Mohamed M. Sharaf and Adel Hasen.
Two hundred day old chicks were used and allotted into equal five groups fed on the basal diet supplemented by 0.0, 25, 50, 75 and 100 mg allicin/Kg diet respectively for five continuous weeks. Dietary allicin supplementation at 25, 50 and 75mg/kg diet significantly (P≤0.05) improved final body weight and total gain by about (6.87%, 12.76% and, 10.13%) and (6.9%, 13.03% and 10.3%) respectively when compared with control broiler chick group. In contrast high level (100mg/Kg) addition of allicin non significantly (P≥0.05) decreased final body weight and total gain of broiler chicks by about 1.13 % and 1.2% respectively, when compared with control one. Allicin supplementation increased feed intake of broiler chicken while, 25, 50 or 75 mg allicin/Kg diet improved feed conversion ratio, protein efficiency ratio, efficiency of energy utilization and performance index when compared with control. In contrast high inclusion rate of allicin deteriorate the mentioned parameters. Allicin supplementation at different levels in broiler chicken ration had no significant effect on WBCs counts, and increased RBCs, Hb%, PCV%, blood serum units and reduced blood serum triglycerides and total cholesterol concentrations when compared with the control. On the other hand, allicin supplementation at 25, 50 or 75 mg/Kg diet improve kidney and liver functions through reduction of blood serum creatnine, GOT, GPT and ALP concentrations when compared with the control, while higher level showed adverse effect. Moreover, it was observed that allicin supplementation improved broiler chicken immune response through increased nutrophil percentage and weight of immune organs when compared with the control one. Dietary allicin supplementation at 25, 50 or 75 mg/Kg diet improved organic matter, crude protein and ether extract digestion, while high inclusion level exhibited lower digestibility percentage and numerically improved dressing percentage of broiler chicken, while had no effect on liver, gizzard, heart weight when compared with the control. Allicin supplementation at 50 mg/Kg diet improved economic efficiency of broiler chick's production.
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Thu, 26 May 2016 13:16:10 GMT -08:00 http://www.scopemed.org/?mno=230326
Pathogenicity of Ten Gallibacterium Anatis Isolates in Commercial Broiler Chickens http://www.scopemed.org/?mno=230324 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Hatem S. Abd El-Hamid, Hany F. Ellakany, Ahmed A. Bekheet, Ahmed R. Elbestawy, and Nahla Mataried.
The aim of the present study was to investigate the incidence of G. anatis infection in commercial broiler and layer chickens in El-behira and Alexandria governorates, Egypt. A total of 64 chicken flocks (53 broiler, 6 layer and 5 breeder flocks) suffering from rales, sneezing and decreased egg production (in layer and breeder flocks) were examined by collecting swabs from trachea, oviduct and ovarian tissues. The molecular identification for the suspected samples, using PCR primers targeted the 16S rRNA (1133fgal) and 23S rRNA (114r) genes revealed 23.43% (15/64) positive isolates of G. anatis. The in-vitro antibiotic sensitivity testing was done and revealed that almost all isolates were sensitive to cefotaxime, norfloxacin, doxycycline and florfenicol. According to the clinical signs, PM lesions and histopathological lesions in commercial broiler chickens infected experimentally there was a significant differences in the pathogenicity in commercial broiler chickens among the 10 isolates used.
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Thu, 26 May 2016 13:08:56 GMT -08:00 http://www.scopemed.org/?mno=230324
Apoptosis in Somatic Cells and Immunological Bioactive Parameters of Cow’s Milk and Their Relation to Subclinical Mastitis http://www.scopemed.org/?mno=230323 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Abeer M. Anwer, Hanaa A. E. Asfour and Inas M. Gamal.
New immunological bioactive parameters are needed for the early detection of subclinical mastitis in dairy animals beside somatic cell count (SCC). A total number of 80 quarter subclinical mastitic (SCM) milk samples and 10 quarter normal milk samples were collected from dairy cows for detection of causing bacteria, some immunological bioactive parameters as well as apoptosis that occur in milk somatic cells (SCs) as a result of SCM and bacterial infection. The main isolated bacteria were Staphylococci that were isolated from all SCM milk especially when SCC exceeded 800x103 cells/ml. SCC were significantly correlated with the severity of SCM as well as the tested immunological parameters eg. haptoglobin (Hp), milk amyloid A (MAA), lactoferrin (Lf), Lysozyme, lactate dehydogenase enzyme (LDH), nitric oxide (NO) and heat shock proteins (HSPs 60) (p < 0.05). The highest significant correlation of these bioactive parameters was detected when SCC exceeded 800x103cells/ml. From the in vitro cultivation of SCs, it was found that they are one of the major sources of immunological parameters that can be used for detection of SCM especially in its early stages. Apoptosis that occurred in SCs was affected by their number as well as the type of bacteria that infected the mammary gland. Furthermore contagious bacteria represented by S.aureus in the in vitro apoptotic assay had the stronger effect on SCs DNA integrity and DNA-fragmentation than the environmental once represented by E.coli.
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Thu, 26 May 2016 12:58:45 GMT -08:00 http://www.scopemed.org/?mno=230323
Abattoir Based Prevalence, Economic Losses and Veterinarians' High-Risk Practices Survey of Bovine Tuberculosis in Mid-Delta of Egypt http://www.scopemed.org/?mno=230322 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Walid Elmonir and Hazem Ramadan.
Bovine Tuberculosis (BTB) is a devastating zoonotic disease with extended influence on animals' productivity, economy and public health. Over three years, a total of 30,841 slaughtered animals (11,389 cows and 19,452 buffaloes) were surveyed for the presence of tuberculous lesions by post-mortem examination in El-Mahalla El-Kubra abattoir in Mid-Delta of Egypt. The overall prevalence of tuberculous lesions among all the slaughtered carcasses was 0.7% (219/30841). Lungs were the most common organ showed tuberculous lesions in cows with a prevalence of 0.24% (27/11389). However, tuberculous lesions were predominately detected in the intestine of buffalo carcasses with a prevalence of 0.45% (87/19452). There was no significant difference associated with either the annual pattern of BTB prevalence or seasonal variation for the two species over the 3 years of the study. Direct economic losses associated with the condemnation of 3105 Kg of meat and 162.5 Kg of liver due to tuberculous lesions was valued as 197,675 EGP (28544.3 USD). Majority of abattoir’s veterinarians injured accidentally during meat inspection at least once a week, yet they never use gloves for protection and some of them won’t cover their wound (42.9%) or even use antiseptics (57.1%). Reported carcasses with BTB lesions may pose a potential public health hazards either for abattoir’s workers or for public consumers when infected carcasses accidentally enter the food chain. Integrated disease surveillance, control of unauthorized slaughtering outside the abattoirs and educational training of veterinarians regarding occupational hazards and protective measures are urgently recommended in attempts to control such infections in the study region.
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Thu, 26 May 2016 12:49:31 GMT -08:00 http://www.scopemed.org/?mno=230322
Effect of Sex, Age and Time of the Day on Vital Parameters of Apparently Healthy West African Dwarf Goats in Abeokuta, Nigeria http://www.scopemed.org/?mno=230321 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Bello, S.A., Akintunde, O. G., Sonibare, A. O., Otesile, E. B..
Four clinically vital parameters (respiratory rate, pulse rate, heart rate and rectal temperature) were evaluated in 54 apparently healthy West African Dwarf goats both in the morning and evening. The mean (and range) of the values obtained for all goats were: respiratory rate, 25±1.9 (15-30) breaths per minute; pulse rate, 86±6.7 (70-102) pulsations per minute; heart rate, 90±6.7 (72-106) beats per minute and rectal temperature, 39.3±0.30C (38.0-41.00C). The sex of goats did not significantly (p>0.05) affect any of the four parameters studied. However, all the vital parameters were significantly (p
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Thu, 26 May 2016 12:42:38 GMT -08:00 http://www.scopemed.org/?mno=230321
West Nile Virus Antibodies in Horse Grooms In Lagos And Ibadan, Southwest Nigeria http://www.scopemed.org/?mno=230320 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Waidi F. Sule, Daniel O. Oluwayelu, Rahamon A.M. Adedokun, Nurudeen Rufai.
Like horses, humans are dead-end hosts and are susceptible to West Nile virus (WNV) infection, which is transmitted through the bite of competent mosquito vectors. We therefore hypothesised that the horse grooms that daily manage polo horses in Lagos and Ibadan, southwest Nigeria don’t have serologic evidence of exposure to this virus. Blood samples were aseptically collected from 13 and 12 apparently healthy, adult male horse grooms in Onosa horse ranch, Lagos and Eleyele Polo club, Ibadan, respectively. Sera from the samples were tested for the presence of WNV-specific antibodies using ELISA and data obtained were analysed using Chi-square and t-tests. The age of the participants ranged from 18.0 - 46.0 years (mean age: 31.1 years), with mean ages of grooms in Lagos and Ibadan being comparable (p=0.34). An overall WNV antibody prevalence rate of 56.0% (95% CI: 36.5 – 75.5%) was observed among the 25 grooms. While the 46.2% and 66.7% prevalence rates for Lagos and Ibadan grooms respectively were comparable (p=0.30), the latter had more than twice likelihood (odds ratio = 2.4) of being seropositive than the former. We concluded that about 50.0% of the horse grooms lacked serologic evidence of exposure to WNV and were therefore susceptible in the event of a WNV outbreak. Continual surveillance for WNV among horse grooms and other occupationally exposed individuals in southwest Nigeria is recommended for prompt detection of new infections.
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Thu, 26 May 2016 12:35:52 GMT -08:00 http://www.scopemed.org/?mno=230320
Prevalence and Antibiotic Susceptibility of Aerobic Bacterial Flora of the Skin, Wound and Anterior Vagina of Sheep, Goats and Dogs in Maiduguri, Nigeria http://www.scopemed.org/?mno=230316 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Yachilla M. Bukar-kolo, Innocent D. Peter, Muhammad M. Bukar, Abubakar A. Muhammad, Ilya J. Ayok.
The present study was designed to investigate antibiotic sensitivity of bacteria isolated from the skin, wound and anterior vagina of sheep, goats and dogs and their susceptibility to graded concentrations of Penicillin and Streptomycin combination (Penstrep®) in Maiduguri, Nigeria. A total of 70 female animals (31 ewes, 24 does and 15 bitches) were sampled. Six bacteria types (Bacillus subtillis, Escherichia coli, Staphylococcus aureus, Proteus spp, Streptococcus pyogenes, Corynebacteria spp) were obtained from 179 isolates with no significant differences (p>0.05) between the proportion of these bacteria isolates amongst the animals studied. Eighty-seven bacterial isolates from skin and wound comprising of 7 bacteria types (Bacillus subtillis, Escherichia coli, Staphylococcus aureus, Proteus spp, Streptococcus pyogenes, Corynebacteria spp, Pseudomonas aeroginosa) were also found. The proportion of these bacteria isolates among the ewes, does and bitches were also not significantly different (p>0.05). All the bacteria were sensitive to Gentamycin and Ofloxacin. In addition, all the bacteria except Corynebacterium spp were sensitive to Ciprofloxacin. However, the bacteria were resistant to Cefuroxine, Ampicillin, Amoxycillin and Floxapen. It was found that B. subtilis was sensitive to Penstrep® at concentrations of 10 mg/ml. Moreso, E. coli, S. aureus, Proteus spp, P. aeruginosa, S. pyogenes and Corynebacterium spp were also sensitive to Penstrep®. However, S. pyogenes and Corynebacterium spp were not sensitive to Penstrep® at 10 mg/ml and the zone of inhibition at 20 mg/ml was least among the bacteria isolated. It was concluded that multi-drug resistant bacteria were resident on the skin, wounds and anterior vagina of the domestic animals studied and there is a need to sensitize animal health professionals in the region of their presence.
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Thu, 26 May 2016 12:23:10 GMT -08:00 http://www.scopemed.org/?mno=230316
Prevalence and Antibiotic Susceptibility of Aerobic Bacterial Flora of the Skin, Wound and Anterior Vagina of Sheep, Goats and Dogs in Maiduguri, Nigeria http://www.scopemed.org/?mno=230315 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Yachilla M. Bukar-kolo, Innocent D. Peter, Muhammad M. Bukar, Abubakar A. Muhammad, Ilya J. Ayok.
The present study was designed to investigate antibiotic sensitivity of bacteria isolated from the skin, wound and anterior vagina of sheep, goats and dogs and their susceptibility to graded concentrations of Penicillin and Streptomycin combination (Penstrep®) in Maiduguri, Nigeria. A total of 70 female animals (31 ewes, 24 does and 15 bitches) were sampled. Six bacteria types (Bacillus subtillis, Escherichia coli, Staphylococcus aureus, Proteus spp, Streptococcus pyogenes, Corynebacteria spp) were obtained from 179 isolates with no significant differences (p>0.05) between the proportion of these bacteria isolates amongst the animals studied. Eighty-seven bacterial isolates from skin and wound comprising of 7 bacteria types (Bacillus subtillis, Escherichia coli, Staphylococcus aureus, Proteus spp, Streptococcus pyogenes, Corynebacteria spp, Pseudomonas aeroginosa) were also found. The proportion of these bacteria isolates among the ewes, does and bitches were also not significantly different (p>0.05). All the bacteria were sensitive to Gentamycin and Ofloxacin. In addition, all the bacteria except Corynebacterium spp were sensitive to Ciprofloxacin. However, the bacteria were resistant to Cefuroxine, Ampicillin, Amoxycillin and Floxapen. It was found that B. subtilis was sensitive to Penstrep® at concentrations of 10 mg/ml. Moreso, E. coli, S. aureus, Proteus spp, P. aeruginosa, S. pyogenes and Corynebacterium spp were also sensitive to Penstrep®. However, S. pyogenes and Corynebacterium spp were not sensitive to Penstrep® at 10 mg/ml and the zone of inhibition at 20 mg/ml was least among the bacteria isolated. It was concluded that multi-drug resistant bacteria were resident on the skin, wounds and anterior vagina of the domestic animals studied and there is a need to sensitize animal health professionals in the region of their presence.
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Thu, 26 May 2016 12:20:21 GMT -08:00 http://www.scopemed.org/?mno=230315
Some Serum Biochemical Parameters and Acute Phase Proteins in Response to Hypomagnesaemic Tetany in Lactating Cattle http://www.scopemed.org/?mno=230313 2016-05-27T20-57-14Z
Source: Alexandria Journal of Veterinary Sciences
Mahmoud A. Ali and Naglaa A. Gomaa.
Hypomagnesaemic tetany in lactating cattle is a partial dietary deficiency of Mg, but other nutritional or metabolic factors reduce the availability of Mg or increase its body loss. The aim of this study was to determine the biochemical changes and acute phase proteins response in serum of lactating cattle with hypomagnesaemic tetany. This investigation included 11 lactating cows suffered from nervous manifestations such as Nystagmus, muscular tremors, stiffness in gait and convulsion (hypomagnesaemic group) and 15 apparently healthy lactating cows (control group). One blood sample was collected from each cow in hypomagnesaemic and control groups. Total protein, albumin, glucose, liver and muscular enzymes, serum minerals including Ca, Mg, P, K, Na, Cl and acute phase proteins (APPs) including (Haptoglobin, serum amyloid A, C-reactive protein) were measured using commercially available test kits. The results revealed significant decreases in serum total protein, albumin and glucose in hypomagnesamic group. On the other hand, there were significant elevations in both hepatic and muscular enzymes activities including AST, ALT, GGT, CPK, LDH, and AP while serum electrolytes levels were variables. The diseased cattle showed significant hypomagnesaemia, hypocalcemia and hyperkalemia. There were highly significant increases in APPs in diseased cattle. It can be concluded that hypomagnesaemic tetany in cattle is a complex metabolic disorders associated with acute inflammatory response assessed by APPs, which could be used in the future as a prognostic indicators for such disease in cattle.
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Thu, 26 May 2016 12:07:56 GMT -08:00 http://www.scopemed.org/?mno=230313
Psychiatry Journals in Turkey and their Future http://www.scopemed.org/?mno=230288 2016-05-27T20-57-14Z
Source: Anatolian Journal of Psychiatry
Lut Tamam.

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Thu, 26 May 2016 08:49:29 GMT -08:00 http://www.scopemed.org/?mno=230288
Fluoxetine induced trichotillomania in a 7-year- old boy: a case report http://www.scopemed.org/?mno=229825 2016-05-27T20-57-14Z
Source: Anatolian Journal of Psychiatry
Nurullah Bolat, Mesut Yavuz.
Trichotillomania (TTM) is a mental disorder characterized by recurrent hair pulling that result in significant hair loss. Traditionally, serotonin has been the principal neurotransmitter implicated in TTM, however, recent literature strongly supports the role of dopamine in the pathophysiology of TTM. Here we describe 7 year old boy who had fluoxetine-induced trichotillomania. According to the temporal association in time between fluoxetine treatment, the symptoms of trichotillomania and the resolution of symptoms in the absence of fluoxetine, hair pulling was likely fluoxetine induced. It is implicated that serotonin reuptake inhibitors may have facilitative effects on dopamine. We suggest that TTM may be the result of dopamine increase due to fluoxetine treatment. To our knowledge this is the first reported case of antidepressant-induced trichotillomania.
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Thu, 26 May 2016 08:39:45 GMT -08:00 http://www.scopemed.org/?mno=229825
PLANT FLORA OF ALAQAN REGION, TABUK PROVINCE, SAUDI ARABIA http://www.scopemed.org/?mno=230276 2016-05-27T20-57-14Z
Source: THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)
Mohamed M. Moawed.
The present study includes survey and identification of the wild plants in Alaqan region (Tabuk city, Saudi Arabia). Fifteen sites representing different habitats were regularly examined from 2012 to 2014 and its vegetation types were identified. A total of 102 species belonging to 75 genera and 34 families were recorded. The most species rich family was Fabaceae (16 species) followed by the Asteraceae (10 species) and Brassicaceae (eight species); the three families Caryophyllaceae, Poacea and Zygophyllaceae (six species); Resedaceae (five species); both Lamiaceae and Chenopodiaceae (four species) whereas, sixteen families were represented by a single species for each. With respect to the life form, therophytes and chamaephytes are the dominating life forms. Therophytes exhibited the maximum number of species with a percentage of 43.14%, followed by chamaephytes (35.35%); hemicrytophyte (9.8%); Phanerophyte and Geophytes (5.88%), while the least frequent life form was Parasites which represented by three species (2.9%). Saharo-Arabian have the highest share of species (22 species) followed by Saharo-Sindian and Sudano-Zambezian (20 species) while Mediterranean, Saharo-Arabian, Irano –Turanian were represented by 5 species.
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Thu, 26 May 2016 06:41:42 GMT -08:00 http://www.scopemed.org/?mno=230276
Translation of original form of Schutte Emotional Intelligence Test into Turkish and examination of its psychometric properties http://www.scopemed.org/?mno=221802 2016-05-27T20-57-14Z
Source: Anatolian Journal of Psychiatry
Arkun Tatar, Serdar Tok, Merih Tekin Bender, Gaye Saltukoğlu.
Objective: The purpose of the present study is to adopt Schutte Self Report Emotional Intelligence Test (SSREIT-33) into Turkish preserving its original factor structure and number of items by applying the test to a heterogeneous participants group representing general population. It is also intended to conduct a research design in accordance with the main features (psychometric properties) of the presentation study. Methods: A total of 1561 people, 839 female, 722 male, ranging in age from 18 to 78 participated in the study. Results: The criterion validity coefficient of the SSREIT-33 total score with Toronto Alexithymia Scale was determined as -0.63, and it was 0.87 with 41 item Revised Schutte Emotional Intelligence Test; and discriminant validity coefficient of SSREI total score with the factors of Short Form Five Factor Personality Inventory was as follows: 0.48 for openness to experience, -0.28 for emotional stability, 0.39 for conscientiousness, 0.16 for agreeableness and 0.11 for extraversion. The test-retest reliability coefficient for 15 and 30 days applications were 0.81 and 0.78 respectively. Confirmatory factor analysis results revealed acceptable fit index values for single factor structure. Internal consistency coefficient of the test was found as 0.86. Conclusion: The values obtained from the form translated into Turkish in this study were quite similar to the values obtained from SSREI-33 presentation study.
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Thu, 26 May 2016 06:02:02 GMT -08:00 http://www.scopemed.org/?mno=221802
Long-term prognosis of commercially sexually exploited youth in Turkey: brief report http://www.scopemed.org/?mno=221914 2016-05-27T20-57-14Z
Source: Anatolian Journal of Psychiatry
Bengi Semerci, Sarper Taşkıran, Ali Evren Tufan, Işın Şanlı.
Objective: Factors contribute to commercial sexual exploitation of youth (CSEY) and a variety of physical and psychological consequences of CSEY were previously discussed in the literature. The aim of the current study was to determine the long term prognosis of a sample of CSEY in Turkey. Methods: A sample of 108 CSEY (all females) with a mean age of 16.7±2.7 (range=9-22) years living in a specialized center for protection against perpetrators were examined in terms of their sociodemographic variables, previous abuse history, family history and current psychopathology. Identified cases were referred for psychiatric evaluation. We obtained follow-up data 11 years after initial contact from this cohort (mean age 25.9±2.6 at time of follow-up) with the subjects and received information regarding their current functioning. Results: Follow up data revealed that recommendations during psychiatric follow up could not be acted upon. 71.4% of the initial sample (n=76) could be reached via direct or indirect contacts. We found that good/superior functioning was very rare and that one-third of the sample that could be reached still were being sexually exploited. Important considerations derived from the results were discussed in the current article.
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Thu, 26 May 2016 05:58:36 GMT -08:00 http://www.scopemed.org/?mno=221914
The neuroinflammation perspective of depression: reuniting the outstanding mechanisms of the pathophysiology http://www.scopemed.org/?mno=229664 2016-05-27T20-57-14Z
Source: KLINIK PSIKOFARMAKOLOJI BULTENI-BULLETIN OF CLINICAL PSYCHOPHARMACOLOGY
Ceren Sahin, Serdar Dursun, Mesut Cetin, Feyza Aricioglu.
Major Depressive Disorder (MDD) is a serious mental health problem that leads to patients’ disability and has huge impact on social and economic burden to society. The current available medications for the treatment of depression are mainly targeted on enhancing monoamine neurotransmission. However, antidepressant treatments are still lack in high efficacy in many cases which is associated with low treatment response and remission rate. However, the latest knowledge regarding the pathophysiology of depression indicates that depression is developed by highly complex and integrated mechanisms in which monoaminergic deficiency could only be part of. The paradigm is now shifting from monoaminergic hypothesis to significance of other novel mechanisms that could possibly play substantial role for the development of depression in a highly interrelated manner. In fact, neuroinflammation, amongst other mechanisms does seem to be a key pathological component by having impact on certain pathway pathologies including glutamatergic neurotransmission, oxidative processes, neurotropic factors, neurotransmitter metabolism and glucocorticoid functions in the central nervous system (CNS) and in the periphery, thereby triggers the pathological alterations that is thought to contribute to the development of depression. The neuroinflammation comprehends the processes exampled from excessive pro-inflammatory cytokine release to activation of microglia and indolamine 2,3-dioxygenase (IDO) pathway, excessive glutamatergic neurotransmission, hyperactive hypothalamus-pituitary-adrenal (HPA) axis, decreased neurogenesis and synaptic plasticity. In fact, the antidepressant effect of ketamine as a non-competitive N-methyl-D-aspartate (NMDA) receptor antagonist, might be at least partially linked to inflammatory modulations. The significance of inflammation in depression is not only mentioned by the literature of basic researches from a mechanistic aspect but also by the possible clinical implications suggested by the clinical reports. Although the exact role of inflammation in depression and its clinical translation have not been determined yet, the inflammation-mediated point of view might provide novel insights for improving the diagnosis at clinic (e.g. inflammatory biomarkers), predicting antidepressant treatment response and thereby re-evaluating the treatment strategy. Moreover, with all that, the inflammation aspect raises the question for the possible significance of utilizing anti-inflammatory approaches in the treatment of depression.
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Thu, 26 May 2016 03:59:11 GMT -08:00 http://www.scopemed.org/?mno=229664
MRI clasification in developmental dysplasia of the hip with reference to soft tissue changes /Gelişimsel kalça displazisinde yumuşak doku değişiklikleri dikkate alınarak yapılan MRG sınıflaması http://www.scopemed.org/?mno=221243 2016-05-27T20-57-14Z
Source: Journal of Turgut Ozal Medical Center
Ozhan Pazarci, Okay Bulut, Seyran Kilinc, Umut Hatay Golge, Zekeriya Oztemur.
Objective: Purpose of the presented prospective study is to assess the pathological soft tissues together and examine their effect on the continuation of reduction in DDH cases treated with closed or open reduction by using MRI technique. Materials and Methods: 46 hips of 34 cases treated between 2003 and 2012 were prospectively studied. MRI was performed on all children after reduction. Kashiwagi MRI classification was made according to the posterior acetabular rim on axial images at the level of triradiate cartilage. As different from Kashiwagi, the group of patientes were divided two subgroups (2A and 2B) according to pathological soft tissue changes on MRI. Results: 27 hips with sharp posterior acetabular rims were involved in Kashiwagi group 1. Sixteen hips with rounded and dysplastic posterior acetabular rims were involved in group 2. In these cases, 9 hips of 6 cases not diagnosed with pathological soft tissue changes obstructing reduction on MRI were included in group 2A. In addition to rounded posterior acetabular rims, 7 hips of 6 cases having at least one of the soft tissue changes such as pulvinar, transverse acetabular ligament, labrum and hypertrophy of ligamentum teres were involved in group 2B. Redislocation was observed after plaster cast in all cases in group 2B and open reduction and salter osteotomy were applied. Three hips with inverted labrum were involved in group 3. Conclusion: Presented study provides more useful information on the course of treatment in cases where Kashiwagi's classification modification and closed/open reduction and immobilization were conducted by taking soft tissue factors obstructing reduction into consideration.
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Thu, 26 May 2016 00:01:40 GMT -08:00 http://www.scopemed.org/?mno=221243
A case of paralytic rabies mimicking Guillain-Barre syndrome / Guillain-Barre sendromunu taklit eden paralitik kuduz olgusu http://www.scopemed.org/?mno=221011 2016-05-27T20-57-14Z
Source: Journal of Turgut Ozal Medical Center
Seher Erdogan, Ahmet Rauf Goktepe, Mehmet Bosnak, Alper Dai.
Rabies is an acute, fatal viral infection of the central nervous system, and one of the oldest zoonotic diseases in human history. A Syrian boy aged 14 had been hospitalized for 2 days with a preliminary diagnosis of Guillain-Barre syndrome because of muscle weakness that spread rapidly to the upper extremities. He was transferred to our unit when the muscle weakness worsened. Respiratory failure developed following admission to the ICU, and the patient was intubated and given ventilator support. Ascending paralysis and albuminocytologic dissociation in the cerebrospinal fluid (CSF) examination suggested a preliminary diagnosis of Guillain-Barre syndrome and plasmapheresis treatment was initiated. No clinical improvement was achieved despite plasmapheresis. On the 10th day of hospitalization, we learned that he had been bitten on the ankle by a stray dog in Syria 2 months ago and had not received prophylaxis. Suspecting that this might be a case of rabies, body fluid samples of the patient were sent to the National Reference Laboratory. The patient died on the 12th day of admission. A postmortem brain biopsy was taken, and Negri bodies were detected. This case report emphasizes that rabies should be considered in the differential diagnosis of patients presenting with paralysis.
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Wed, 25 May 2016 23:51:34 GMT -08:00 http://www.scopemed.org/?mno=221011