Histochemical localisation of oxidative and hydrolytic enzymes was observed in bursa of Fabricius in different age groups of Chicken. The epithelium covering the bursal plicae and lymphoid follicles showed mild to moderate ALP abd ACP activity. The ALP started declining as age advanced. Epithelium covering the bursal plicae, muscular layer and cytoplasm of cortical and medullary lymphocytes of lymphoid follicle showed negative reaction to adenosine triphosphatase activity. In all the age groups studied, there was mild positive reaction to GPDH in tunica serosa and muscular layer. IFE showed strong positive GPDH activity when compared FAE. Epithelium between cortex and medulla and star shaped cells in the medulla of some follicles showed strong positive reaction to GPDH. As age advanced the intensity of enzyme also increased. Compared to all other dehydrogenase, there was a mild reaction to SDH in the bursal tissue. In all the age groups studied, positive reaction was noticed for NADPH diaphorase, ANAE and Cytochrome oxidase activity.
Chicken;Burs of Fabricius;Oxidative and Hydrolytic Enzymes;Localization;Histochemical Methods