Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Review Article



Loop-Mediated Isothermal Amplification Assay (LAMP): A Rapid Tool for Diagnosis of Foodborne and Zoonotic Pathogens: A Review

Ravindra J. Zende; Deepak P. Kshirsagar; Vilas M. Vaidya; Rupesh N. Waghamare; Riddina P. Todankar; Aparna H. Shirke.

Abstract
Loop-mediated isothermal amplification (LAMP) assay was introduced in the year 2000 by Notomi, as a highly sensitive, specific and cost-effective technique for microbial identification. In contrast to the polymerase chain reaction (PCR) technology in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is carried out at a constant temperature and does not require a thermal cycler. LAMP, a simple DNA amplification technique, with its field amenable nature has been used to detect a variety of pathogens including viruses, fungi, bacteria and parasites and in most of the cases it surpasses polymerase chain reaction. This review emphasis on importance of LAMP for successful detection of pathogens from minimally processed samples or in presence of clinical sample matrices. This feature of LAMP may be useful in low resource or field settings where conventional DNA or RNA extraction prior to diagnostic testing may be impractical.

Key words: LAMP;PCR;Foodborne and Zoonotic Pathogens



Share this Article


Advertisement
Oxidants and Antioxidants in Medical Science

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons