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Polymorphism Studies of Kappa-casein (k-CN), Beta-lactoglobulin (β-Lg) and DRB3 Genes in Indian Murrah Buffalo

Hitesh Lamba; Abhishek Pal; Deepak Sharma; Madhu Tiwari; Satyendra Pal Singh; Vijay Pandey.

Abstract
The objective of the present study was to investigate the polymorphism in Kappa-casein (k-CN), Beta-lactoglobulin (β-LG) and DRB3 gene in Murrah buffalo using PCR-RFLP assay. Amplification of DNA sample revealed 350, 247 and 304 bp products using specific primer pairs and digested using HinfI, HaeIII and HaeIII restriction enzymes, respectively. All the screened animals were found monomorphic in nature for k-CN and β-LG genes. Only BB and AB genotype was observed for k-CN and β-LG gene, respectively. While DRB3 gene upon digestion produced five types of genotypes; one of them was of 170, 82 and 52 bp (AA genotype); second of 222 and 82 bp (BB genotype), third of 170 & 134 bp (EE genotype) and heterozygous pattern had 222, 170, 82 and 52 bp bands (AB genotype) and 222, 193, 82 and 29 bp bands (BD genotype). AB genotype was the most frequent in all the screened buffaloes, followed by the AA, EE, BB and BD genotypes. The frequency of DRB3/HaeIII A, B, D and E alleles were 0.422, 0.411, 0.055 and 0.111, respectively. Association study revealed that there was significant difference for total milk yield (TMY) and milk yield in 300 days (MY300) among all the DRB3 genotypes. This study provides a basis for further investigation in other breeds of Indian buffalo.

Key words: Murrah;PCR-RFLP;k-CS;β-Lg;DRB3;Polymorphism;Milk Production Traits



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