Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article

J App Pharm Sci. 2017; 7(2): 067-075


Sequential optimizations of Aspergillus awamori EM66 exochitinase and its application as biopesticide

Ghada E. A. Awad; Walaa A. Abdel Wahab; Mona Hussein; Ahmed El-diwany; Mona A. Esawy.

Abstract
A sequential optimization strategy, based on statistical experimental designs was employed to enhance Aspergillus awamori EM66 exochitinase productivity. First, a 2-level Plackett–Burman design was applied to differentiate between the bioprocess parameters, which significantly influence exochitinase production. The second optimization step was performed using central composite design (CCD), in order to optimize variables amounts that have the highest positive significant effect on exochitinase production. A maximal activity (5998mU/ml) for exochitinase reported approximately 22 fold increase compared to the basal medium activity. Mortality 92%, 86.67% and 65.67% was recorded when the partial pure fungal exochitinase was applied to the diet of the greater wax moth larvae, Galleria mellonella, , the cotton leaf worm, Spodoptera littoralis, and the black cutworm, Agrotis ipsilon, respectively. The results indicated to the importance of Aspergillus awamori EM66 exochitinase as an effective biopesticide.

Key words: Aspergillus awamori;Bio pesticides;Central composite design;Exochitinase



Share this Article


Advertisement
American Journal of Preventive Medicine and Public Health

SUBMIT YOUR ARTICLE NOW


ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Article Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons