Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article

J App Pharm Sci. 2017; 7(3): 123-128


A broad-host range coliphage against a clinically isolated E. coli O157: isolation and characterization

Abdollah Ghasemian; Maryam Bavand; Zahra Moradpour.

Abstract
Escherichia coli O157 is one of the common and problematic pathogens, particularly in developing countries. To isolate a phage against this pathogen, a total of 32 water samples were enriched for phage and 2 of these samples were found to contain lytic phages that grow on and kill enterohaemorrhagic E. coli serotype O157. Following the primary evaluation, the most efficient phage was chosen for further characterization. The multiplicity of infection, morphology, killing efficiency and bacterial host range were determined under controlled conditions in the laboratory. Isolated phage was designated gBSN-MGB13. According to transmission electron microscopy, this lytic phage morphologically belonged to the myoviridae family. Based on phage efficiency test, a multiplicity of infection of 5 logs of gBSN-MGB13 resulted in 50% reduction in viable bacterial cell count after 20 min incubation in 37 °C without shaking. Since gBSN-MGB13 is a broad-host range phage and effective against several pathogenic species, E. coli O157 as well as Proteus vulgaris and Pseudomonas aeruginosa, its applications could be investigated in complex infections as combinatory therapy. This is an exciting aspect of phage therapy.

Key words: E. coli O157;antibiotic resistance;phage therapy;siphoviridae



Share this Article


Advertisement
Journal of Apitherapy

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons