Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Article

Characterization and potential antimicrobial effect of novel chitooligosaccharides against pathogenic microorganisms

Sanaa T. El-Sayed; Amal M. Ali; El-Sayed M. El-Sayed; Wafaa G. Shousha; Nagwa I. Omar.

Chitosan has antimicrobial activities against a wide range of food-borne microorganisms. It was poorly soluble in neutral solution, which makes them difficult to use in food and biomedical applications. While chitooligosaccharides were fairly soluble in an aqueous solution and consequently facilitated their utilization in industrial application. Therefore, enzymatic production of chitooligosaccharides from chitosan, previously, was carried out using immobilized pepper chitosanase. Chitooligosaccharides I, II, III and IV groups were prepared with molecular weight > 100, 100-10, 10-1 and < 1 KDa, respectively. Their particles were compared with chitosan by scanning electron microscope (SEM). Their degree of fissure and cracking were increased with decrease the molecular weight. The rod shape appear in IV group, indicated to the presence of disaccharides (chitobioses). Ultraviolet spectrum (UV) showed that IV group had different absorbance at wave length ranged from (309-348 nm) than that of the other groups. The main difference in Fourier transform infra red spectroscopy (FT-IR) of the four groups and native chitosan were observed in the range of the wave number 3750-3000 Cm-1. Potential antimicrobial activity (in vitro) of chitooligosaccharides groups were evaluated against some selected pathogenic microorganism and were compared with that of chitosan. They were measured using the standard method of diffusion disc plates on agar (50 µg/ml). Our results clearly indicated that the antimicrobial activities depend mainly on the type of microorganism tested. Chitooligosaccharides had antimicrobial activity as follows: groups (I, II, III) against B. cereus, group (I, III, IV) against B. subtilis, group (II, IV) against Staphylococcus aureus, group (II) against Pseudomonas aeruginosa, group (I, II, IV) against Candida albicans and group (III) against Saccharomyces chevaliae.

Key words: Chitooligosaccharides;Scanning electron microscope (SEM);Ultraviolet spectrum (UV);Fourier transform infra red spectroscopy (FT-IR);Pathogenic microorganism and Antimicrobial activity

Share this Article

Journal of Molecular Pathophysiology


ScopeMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons