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Preliminary Assessment of Within-Ecotype Genetic Diversity at the Haemoglobin Locus in the Tiv Local Chickens in Makurdi, Nigeria

Henry Okechukwu Ukwu; Deborah Onyinye Ezechukwu; Francis Odache; Vincent Owoicho Okopi; Moses Adah Egbere; Victoria O. Sunday.

This experiment was designed to assess within-ecotype genetic diversity at the haemoglobin (Hb) locus in the Tiv local chickens of Nigeria in Makurdi. Eighty-two (82) blood samples were collected from each bird using needles and vials (SARSTEDT MonovetteĀ®). Haemoglobin genotypes were determined using cellulose acetate electrophoresis. Data were analyzed using Population Genetics Simulation (PopGen.S2) software. The result revealed three haemoglobin genotypes (HbAA, HbAB and HbBB) controlled by two co-dominant haemoglobin alleles HbA and HbB in the Tiv local chicken population in Makurdi. Genotype frequencies of HbAA, HbAB and HbBB were 0.44, 0.32 and 0.24, respectively, in the entire chicken population. The gene frequencies of HbA and HbB observed in this study were 0.60 and 0.40, respectively in the entire population. There was moderate heterozygosity (0.48) at the haemoglobin locus in the local chicken population in Makurdi, which is an advantage to the local chicken population.

Key words: Alleles;diversity;genotype;haemoglobin locus;local chicken

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