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Sex Related Hospital Prevalence of Theileria annulata Infection Confirmed by Nested Polymerase Chain Reaction in Cattle-calves in Bikaner District of Rajasthan

Pavan Goyal.

Abstract
One hundred cattle-calves out of which 58 female and 42 male cattle-calves were screened for Theileria annulata infection during October-2015 to September-2016 brought to Teaching Veterinary Clinical Complex, College of Veterinary and Animal Science, Bikaner. Blood samples were collected in ethylene diamine tetra acetic acid (EDTA @1mg/ml) vacutainers from all the suspected cattle-calves for genomic DNA isolation and subjected for confirmatory diagnosis by nested polymerase chain reaction (nPCR). Prevalence of Theileria annulata infection was 44.83 per cent in female cattle-calves (26/58) while 35.71 per cent in male cattle-calves (15/42). Thus, the prevalence was higher in female as compared to male cattle-calves.

Key words: Cattle-Calves;Prevalence;DNA;Theileria annulata;nPCR



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