Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Research

. 2017; 1(2): 52-60


The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro

Soad Nady; Irene S Gmail; Fatma Elzahraa H Salem; Rabab Salama; Asmaa Ezz.

Abstract
Background: Pollen and pollen products have a long history of use in traditional herbal medicine and have been reported to display a wide range of antimicrobial, antioxidative, anti-inflammatory, antioxidant, and hepato-protective activities. This study aimed to investigate the role of date palm pollen (DPP) ethanolic extract on the formation of granuloma induced by Schistosoma mansoni soluble egg antigen (SEA) in vitro.
Methodology: Peripheral blood mononuclear cells (PBMCs) were isolated from 20 S. mansoni monoinfected, 50 Hepatitis C virus/S. mansoni coinfected patients and 25 healthy individuals. The isolated PBMCs were used for in vitro granuloma induction using SEA conjugated polyacrylamide beads. Different concentrations of DPP ethanolic extract (10 mg/mL, 20 mg/mL, 30 mg/mL, and 40 mg/mL) were added to the granuloma at the first day, then the sizes of granulomas were measured by granuloma index (GI) that was assessed by quantitation of cellular reactivity around the beads after 3, 7, and 14 days.
Results: Treatment of granuloma induced by SEA conjugated polyacrylamide beads with the optimum concen-tration of DPP ethanolic extract (30 mg/mL) resulted in significant (P < 0.05) reduction of GI using PBMCs iso¬lated from Schistosoma monoinfected, hepatitis C virus/Schistosoma coinfected patients or healthy individuals at all the experimental time intervals.
Conclusion: DPP suppressed the effect of SEA and prevented granuloma formation. The results of the present study suggest that DPP could be considered a promising source of new natural anti-inflammatory herbal med¬icine against the S. mansoni associated granulomatous inflammation.

Key words: Date palm pollen;Schistosoma mansoni;granuloma;soluble egg antigen



Share this Article


Advertisement
Journal of Behavioral Health

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons