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Original Article

J App Pharm Sci. 2017; 7(9): 207-212

Chemical Composition and Antimicrobial Activity of the Essential Oil of the Leaves of Cupressus macrocarpa Hartweg. ex Gordon

Amal M. Saad; Magdy M.D. Mohammed; Mosad A. Ghareeb; Wafaa S. Ahmed; Mohamed A. Farid.

Qualitative (GC-MS) and quantitative (GC-FID) analyses of the essential oil (EO) isolated from the leaves of Cupressus macrocarpa revealed the presence of fifteen components, and representing 86.29% of the total oil composition. Their identification were based on their retention times and mass spectral fragmentation patterns. α-terpineol (19.01%), camphenilone (9.78%), elemol (8.92%), 2-tridecanone (8.75%), α-terpinyl acetate (8.62%), 2-pentyl-2-cyclopenten-1-one (6.90%) and β-bisabolol (5.83%) represent major components. Agar diffusion method was conducted to test the antimicrobial activity of the EO against eight pathogenic strains, including gram (+ve) and (-ve) bacteria and fungi. The obtained results showed inhibition zones with 10.5-21mm diameters, compared to Streptomycin.

Key words: Monterey Cypress;Cupressaceae;Volatile constituents;GC/MS;GC/FID;Antibacterial activity;Antifungal activity;MIC

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